PXD021211

PXD021211 is an original dataset announced via ProteomeXchange.

Title	Structure of TFIIK for phosphorylation of CTD of RNA polymerase II
Description	TFIIH is a 10-protein complex that is conserved through out eukaryotes. TFIIH has two primary cellular functions: transcription initiation and nucleotide excision repair (NER). In transcription initiation, TFIIH acts as a structural scaffold, phosphorylates the RNA polymerase II (pol II) C-terminal domain (CTD) and translocates promoter DNA through the pol II active site to facilitate start site selection. In NER, again is a structural scaffold, opens a bubble around damaged DNA and scans the damaged strand for bulky lesions. In yeast (Saccharomyces cerevisiae), TFIIH is composed of the two helicases Ssl2 and Rad3, the scaffolding subunits Tfb1, Tfb2, Tfb4 and Ssl1 and the kinase subunits Kin28, Ccl1 and Tfb3.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:23:46.249.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hee Jong Kim
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-31 01:28:32	ID requested
1	2021-07-06 22:35:59	announced
⏵ 2	2024-10-22 05:23:47	announced	2024-10-22: Updated project metadata.

van Eeuwen T, Li T, Kim HJ, Gorbea Col, ó, n JJ, Parker MI, Dunbrack RL, Garcia BA, Tsai KL, Murakami K, Structure of TFIIK for phosphorylation of CTD of RNA polymerase II. Sci Adv, 7(15):(2021) [pubmed]

10.1126/sciadv.abd4420;

submitter keyword: transcription,TFIIK, phosphorylation

Benjamin Aaron Garcia
contact affiliation	University of Pennsylvania
contact email	bgarci@pennmedicine.upenn.edu
lab head
Hee Jong Kim
contact affiliation	University of Pennsylvania
contact email	heejong@heejong.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD021211

PRIDE project URI

• PRIDE
  1. PXD021211
     1. Label: PRIDE project
     2. Name: Structure of TFIIK for phosphorylation of CTD of RNA polymerase II