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PXD021206 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAssessing the short and long-term impacts of protrusion induction on the proteome of MDA-MB231 cells
DescriptionTo assess the impact of protrusion induction on the proteome of mesenchymal-like migratory cells, MDA-MB231 breast cancer cells were seeded on top of collagen-I coated 3 micron transwell filters (corning). No media was added to the bottom chamber and cells were allowed to adhere to filter overnight. The next day, the media on top of cells was refreshed, and the filters were either left with no media in the bottom chamber (closed pores), or with media also added to the bottom chamber (open pores) in order to allow formation of protrusions. Cells were allowed to form protrusions for 2 or 24 hrs, before being lysed and analysed by mass spectrometry, using TMT mediated quantitative proteomics.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaria Dermit Salazar
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-31 01:09:33ID requested
12020-10-19 22:24:22announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: TMT
Contact List
Faraz Mardakheh
contact affiliationCentre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, Charterhouse square, London EC1M 6BQ, The United Kingdom.
contact emailf.mardakheh@qmul.ac.uk
lab head
Maria Dermit Salazar
contact affiliationqmul
contact emailmariaprideproteomics@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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