PXD021205

PXD021205 is an original dataset announced via ProteomeXchange.

Title	Assessment of nascent protein accumulation in different subcellular fractions following protrusion induction
Description	To assess if newly synthesised ribosomal proteins participate in canonical ribosome biogenesis in the nucleus, following their enhanced translation upon protrusion induction, Light (L) SILAC labelled MDA-MB231 breast cancer cells were seeded on top of collagen-I coated 3 micron transwell filters (corning). No media was added to the bottom chamber and cells were allowed to adhere to filter overnight. The next day, the media on top of cells was replaced with either medium (M) (closed pores) or heavy (H) SILAC media to pulse label newly synthesised proteins. H media was also added to the bottom chamber (open pores) in order to allow formation of protrusions in the H condition. Cells were pulsed for 4 hrs before being lysed and H and M pulsed samples were mixed together. Mixed lysates were then subjected to subcellular fractionation by serial solubilization (cytosol, membrane and nucleus) using Pierce subcellular protein fractionation kit (78840).
HostingRepository	PRIDE
AnnounceDate	2020-10-20
AnnouncementXML	Submission_2020-10-19_22:26:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Dermit Salazar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-31 01:08:48	ID requested
⏵ 1	2020-10-19 22:26:08	announced

Dataset with its publication pending

submitter keyword: pulsed-SILAC

pSILAC

iBAQ

protrusions

MDA-MB231

Orbitrap

Faraz Mardakheh
contact affiliation	Centre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, Charterhouse square, London EC1M 6BQ, The United Kingdom.
contact email	f.mardakheh@qmul.ac.uk
lab head
Maria Dermit Salazar
contact affiliation	qmul
contact email	mariaprideproteomics@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD021205

PRIDE project URI

• PRIDE
  1. PXD021205
     1. Label: PRIDE project
     2. Name: Assessment of nascent protein accumulation in different subcellular fractions following protrusion induction