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PXD021205 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAssessment of nascent protein accumulation in different subcellular fractions following protrusion induction
DescriptionTo assess if newly synthesised ribosomal proteins participate in canonical ribosome biogenesis in the nucleus, following their enhanced translation upon protrusion induction, Light (L) SILAC labelled MDA-MB231 breast cancer cells were seeded on top of collagen-I coated 3 micron transwell filters (corning). No media was added to the bottom chamber and cells were allowed to adhere to filter overnight. The next day, the media on top of cells was replaced with either medium (M) (closed pores) or heavy (H) SILAC media to pulse label newly synthesised proteins. H media was also added to the bottom chamber (open pores) in order to allow formation of protrusions in the H condition. Cells were pulsed for 4 hrs before being lysed and H and M pulsed samples were mixed together. Mixed lysates were then subjected to subcellular fractionation by serial solubilization (cytosol, membrane and nucleus) using Pierce subcellular protein fractionation kit (78840).
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaria Dermit Salazar
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-31 01:08:48ID requested
12020-10-19 22:26:08announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: pulsed-SILAC
Contact List
Faraz Mardakheh
contact affiliationCentre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, Charterhouse square, London EC1M 6BQ, The United Kingdom.
contact emailf.mardakheh@qmul.ac.uk
lab head
Maria Dermit Salazar
contact affiliationqmul
contact emailmariaprideproteomics@gmail.com
dataset submitter
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Dataset FTP location
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