PXD021204

PXD021204 is an original dataset announced via ProteomeXchange.

Title	Analysis of LARP6 depletion impact on the proteome of MDA-MB231 breast cancer cells
Description	We analysed the impact of LARP6 depletion on the proteome of actively growing MDA-MB231 breast cancer cells by SILAC. For this purpose, Light (L) SILAC-labelled MDA-MB231 cells were treated with non-targeting control (NT) or two independent LARP6 siRNA (18i & 97i) for 72 hrs, before lysis in 4% SDS, 100mM Tris/HCl pH 7.5. In parallel, Heavy (H)SILAC labelled non-transfected MDA-MB231 cells were grown and lysed similarly. Each L labeled lysate was then mixed with an equal amount of H labelled lysate. Mixing of samples to the same H standard therefore allowed cross-comparison of different siRNA treatments from separate runs.
HostingRepository	PRIDE
AnnounceDate	2020-10-20
AnnouncementXML	Submission_2020-10-19_22:28:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Dermit Salazar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-31 01:07:58	ID requested
⏵ 1	2020-10-19 22:29:00	announced

Dataset with its publication pending

submitter keyword: LARP6

SILAC

MDA-MB231

Breast cancer

Orbitrap

Faraz Mardakheh
contact affiliation	Centre for Cancer Cell & Molecular Biology Barts Cancer Institute Queen Mary University of London Charterhouse Square London EC1M 6BQ The United Kingdom.
contact email	f.mardakheh@qmul.ac.uk
lab head
Maria Dermit Salazar
contact affiliation	qmul
contact email	mariaprideproteomics@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD021204

PRIDE project URI

• PRIDE
  1. PXD021204
     1. Label: PRIDE project
     2. Name: Analysis of LARP6 depletion impact on the proteome of MDA-MB231 breast cancer cells