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PXD021203 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePulsed-SILAC analysis of protein translation rate changes upon protrusion formation
DescriptionTo assess the impact of protrusion formation on protein synthesis rates on a global scale, we carried out a pulsed-SILAC (pSILAC) analysis in MDA-MB231 cells grown with or without protrusions for 1, 2, 4, and 8 hrs. Briefly, light SILAC labelled cells were seeded on top of 2x 3 micron transwell filters without any media added to the bottom chamber. Cells were allowed to attach to the filter overnight, and the next day the media on the top was changed to medium (M) or heavy(H) SILAC DMEM. At the same time, the H SILAC media was also added to the bottom chamber of the transwell with H media on the top to induce protrusions. Cells were then allowed to form protrusions for 1, 2, 4, or 8 hrs, before being lysed and mixed with the M labelled lysates (cells without protrusions) from the same timepoint. The experiment was then repeated with switched SILAC labelling.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaria Dermit Salazar
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-31 01:07:13ID requested
12020-10-19 22:25:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Pulsed-SILAC
Contact List
Faraz Mardakheh
contact affiliationCentre for Cancer Cell & Molecular Biology Barts Cancer Institute Queen Mary University of London Charterhouse Square London EC1M 6BQ The United Kingdom.
contact emailf.mardakheh@qmul.ac.uk
lab head
Maria Dermit Salazar
contact affiliationqmul
contact emailmariaprideproteomics@gmail.com
dataset submitter
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Dataset FTP location
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