PXD021199

PXD021199 is an original dataset announced via ProteomeXchange.

Title	PLEKHM1 is a direct target for both mTOR and MAPK pathways
Description	The lysosome is a cellular signalling hub at the point of convergence of endocytic and autophagic pathways, where the contents are degraded and recycled. Previously, we identified the adaptor protein PLEKHM1, which facilitates the fusion of endocytic and autophagic vesicles with the lysosome. It is unclear how the function of PLEKHM1 at the lysosome is controlled. Herein, we show that PLEKHM1 co-precipitates with mTOR and its regulatory partners. PLEKHM1 is directly phosphorylated by mTOR at a proline-directed dual serine motif. Using a phospho-specific antibody that recognizes PLEKHM1 when phosphorylated at Serine432/Ser435, we show that the same motif is a direct target for ERK2-mediated phosphorylation in a growth factor-dependent manner. This dual regulation of PLEKHM1 at a highly conserved region points to a convergence of both growth factor- and amino acid-sensing pathways, placing PLEKHM1 at a critical juncture of two major cellular metabolic pathways.
HostingRepository	PRIDE
AnnounceDate	2021-01-19
AnnouncementXML	Submission_2021-01-18_23:43:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marina Hoffmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-31 00:33:22	ID requested
⏵ 1	2021-01-18 23:43:32	announced

Gubas A, Karantanou C, Popovic D, Tascher G, Hoffmann ME, Platzek A, Dawe N, Dikic I, Krause DS, McEwan DG, The endolysosomal adaptor PLEKHM1 is a direct target for both mTOR and MAPK pathways. FEBS Lett, 595(7):864-880(2021) [pubmed]

submitter keyword: Human, SILAC, GFP-Trap pull-down, autophagy, phosphorylation

Ivan Dikic
contact affiliation	Institute of Biochemistry II, Faculty of Medicine, Goethe University Frankfurt, Germany
contact email	dikic@biochem2.uni-frankfurt.de
lab head
Marina Hoffmann
contact affiliation	institute of biochemistry 2 (IBC2)
contact email	hoffmann@med.uni-frankfurt.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021199
     1. Label: PRIDE project
     2. Name: PLEKHM1 is a direct target for both mTOR and MAPK pathways