PXD021198

PXD021198 is an original dataset announced via ProteomeXchange.

Title	Protein stability changes reveal targets of pyrithione-induced Cu toxicity in E. coli
Description	The biological activities of metals and ionophores have brought them to the forefront as potent antimicrobial and anticancer agents. However, the biological mechanisms behind these activities are not well understood. This work describes the first utilization of proteome-wide measurements of protein folding stability in combination with protein expression level analyses to identify the protein targets of metals and provides insight into ionophore-induced Cu toxicity in E. coli. Stability and expression level profiles were generated on the proteins in cell lysates derived from E. coli cells exposed to copper in the absence and presence of two ionophores, the antimicrobial agent pyrithione and a β-lactamase-activated prodrug of pyrithione, PcephPT. The differential profiles enabled the effects of Cu to be distinguished from the effects the ionophores. The relatively large number of differentially stabilized proteins identified here were especially informative, and revealed both established and novel mechanisms of action for Cu-induced death.
HostingRepository	PRIDE
AnnounceDate	2020-12-08
AnnouncementXML	Submission_2020-12-08_12:17:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nancy Corsi
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-30 23:52:46	ID requested
⏵ 1	2020-12-08 12:17:36	announced

Dataset with its publication pending

submitter keyword: E. coli, Cu toxicity, Pyrithione, protein stability

Michael C. Fitzgerald
contact affiliation	Professor of Chemistry Duke University, Department of Chemistry
contact email	michael.c.fitzgerald@duke.edu
lab head
Nancy Corsi
contact affiliation	Duke University
contact email	njw23@duke.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021198
     1. Label: PRIDE project
     2. Name: Protein stability changes reveal targets of pyrithione-induced Cu toxicity in E. coli