PXD021197

PXD021197 is an original dataset announced via ProteomeXchange.

Title	COVID-19 proteome profiling reveals resolution of inflammatory pathways and respiratory distress after DNase treatment
Description	Severe COVID-19 can result in pneumonia and acute respiratory failure. Accumulation of mucus in the airways is a hall mark of the disease and can result in hypoxemia. Here, we show that quantitative proteome analysis of the sputum from severe COVID-19 patients reveal high levels of neutrophil extracellular trap(s) (NETs) components, which was confirmed by microscopy. Degrading pulmonary NETs using clinically approved aerosolized recombinant human DNase (rhDNase/Pulmozyme) improved pulmonary function, reversed hypoxemia, and aided in the rapid recovery of severely ill COVID-19 patients. Immunofluorescence and proteome analysis of sputum and blood plasma samples after treatment revealed a marked reduction of NETs and a set of statistically significant proteome changes that indicate local reduction of haemorrhage, plasma leakage and inflammation in the airways, and a reversion of the systemic inflammatory state in the blood plasma. Taken together, the results show that NETs contribute to acute respiratory failure in COVID-19 and that degrading NETs may reduce dependency on external high flow oxygen therapy. Targeting NETs may have significant therapeutic implications in COVID-19 disease and warrants further studies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:23:39.668.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christofer Karlsson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; L-citrulline
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-30 23:43:24	ID requested
1	2021-07-05 09:42:31	announced
⏵ 2	2024-10-22 05:23:40	announced	2024-10-22: Updated project metadata.

10.1016/j.mcpro.2021.100113;

Fisher J, Mohanty T, Karlsson CAQ, Khademi SMH, Malmstr, ö, m E, Frigyesi A, Nordenfelt P, Malmstrom J, Linder A, Proteome Profiling of Recombinant DNase Therapy in Reducing NETs and Aiding Recovery in COVID-19 Patients. Mol Cell Proteomics, 20():100113(2021) [pubmed]

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: human plasma DIA SWATH-MS covid-19 HF-X

Johan Malmström
contact affiliation	Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, Division of Infection Medicine, Lund, Sweden
contact email	johan.malmstrom@med.lu.se
lab head
Christofer Karlsson
contact affiliation	Lund University
contact email	Christofer.Karlsson@med.lu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021197
     1. Label: PRIDE project
     2. Name: COVID-19 proteome profiling reveals resolution of inflammatory pathways and respiratory distress after DNase treatment