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PXD021192

PXD021192 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic profiling of two distinct populations of extracellular vesicles isolated from human seminal plasma indicates their biogenesis by prostate epithelial cells and potential as biomarker for prostate cancer
DescriptionBody fluids contain many populations of extracellular vesicles (EV) that differ in size, cellular origin, molecular composition, and biological activities. EV in seminal plasma are in majority originating from prostate epithelial cells, and hence are also referred to as prostasomes. Nevertheless, EV are also contributed by other accessory sex glands, as well as by the testis and epididymis. In a previous study, we isolated EV from seminal plasma of vasectomized men, thereby excluding contributions from the testis and epididymis, and identified two distinct EV populations with diameters of 50 and 100 nm respectively, each with specific marker proteins. In the current study, we comprehensively analyzed the protein composition of these two EV populations using quantitative Liquid Chromatography-Mass Spectrometry (LC-MS/MS). In total 1558 proteins were identified. Of these, ~45% was found only in the isolated 100 nm EV, 1% only in the isolated 50 nm EV, and the remainder in both 100 nm and 50 nm EV. Gene ontology (GO) enrichment analysis suggest that both originate from the prostate, but with distinct biogenesis pathways. Finally, 9 proteins with known prostate specific expression and alternate expression levels in prostate cancer tissue were identified in isolated EV. These data may have potential for the discovery of EV associated prostate cancer biomarkers in blood.
HostingRepositoryPRIDE
AnnounceDate2022-02-16
AnnouncementXMLSubmission_2022-02-16_08:02:54.331.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHarmjan Vos
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-28 07:05:46ID requested
12022-02-16 08:02:55announced
Publication List
Zhang X, Vos HR, Tao W, Stoorvogel W, Proteomic Profiling of Two Distinct Populations of Extracellular Vesicles Isolated from Human Seminal Plasma. Int J Mol Sci, 21(21):(2020) [pubmed]
Keyword List
submitter keyword: extracellular vesicles
prostasomes
proteomic
subtypes
seminal fluid
Contact List
Willem Stoorvogel
contact affiliationDepartment of Biomolecular Health Sciences, Faculty of Veterinary Science, Utrecht University, Utrecht, The Netherlands
contact emailw.stoorvogel@uu.nl
lab head
Harmjan Vos
contact affiliationUniversity Medical Center Utrecht Dept. Molecular Cancer Research
contact emailh.r.vos-3@umcutrecht.nl
dataset submitter
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Dataset FTP location
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