PXD021192

PXD021192 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of two distinct populations of extracellular vesicles isolated from human seminal plasma indicates their biogenesis by prostate epithelial cells and potential as biomarker for prostate cancer
Description	Body fluids contain many populations of extracellular vesicles (EV) that differ in size, cellular origin, molecular composition, and biological activities. EV in seminal plasma are in majority originating from prostate epithelial cells, and hence are also referred to as prostasomes. Nevertheless, EV are also contributed by other accessory sex glands, as well as by the testis and epididymis. In a previous study, we isolated EV from seminal plasma of vasectomized men, thereby excluding contributions from the testis and epididymis, and identified two distinct EV populations with diameters of 50 and 100 nm respectively, each with specific marker proteins. In the current study, we comprehensively analyzed the protein composition of these two EV populations using quantitative Liquid Chromatography-Mass Spectrometry (LC-MS/MS). In total 1558 proteins were identified. Of these, ~45% was found only in the isolated 100 nm EV, 1% only in the isolated 50 nm EV, and the remainder in both 100 nm and 50 nm EV. Gene ontology (GO) enrichment analysis suggest that both originate from the prostate, but with distinct biogenesis pathways. Finally, 9 proteins with known prostate specific expression and alternate expression levels in prostate cancer tissue were identified in isolated EV. These data may have potential for the discovery of EV associated prostate cancer biomarkers in blood.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_08:02:54.331.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harmjan Vos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-28 07:05:46	ID requested
⏵ 1	2022-02-16 08:02:55	announced

Zhang X, Vos HR, Tao W, Stoorvogel W, Proteomic Profiling of Two Distinct Populations of Extracellular Vesicles Isolated from Human Seminal Plasma. Int J Mol Sci, 21(21):(2020) [pubmed]

submitter keyword: extracellular vesicles

prostasomes

proteomic

subtypes

seminal fluid

Willem Stoorvogel
contact affiliation	Department of Biomolecular Health Sciences, Faculty of Veterinary Science, Utrecht University, Utrecht, The Netherlands
contact email	w.stoorvogel@uu.nl
lab head
Harmjan Vos
contact affiliation	University Medical Center Utrecht Dept. Molecular Cancer Research
contact email	h.r.vos-3@umcutrecht.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021192

PRIDE project URI

• PRIDE
  1. PXD021192
     1. Label: PRIDE project
     2. Name: Proteomic profiling of two distinct populations of extracellular vesicles isolated from human seminal plasma indicates their biogenesis by prostate epithelial cells and potential as biomarker for prostate cancer