PXD021186

PXD021186 is an original dataset announced via ProteomeXchange.

Title	ATP-Citrate lyase fuels axonal transport across species
Description	Microtubule (MT)-based transport is an evolutionary conserved processed finely tuned by posttranslational modifications. Among them, tubulin acetylation, which is catalyzed by the α-tubulin N-acetyltransferase 1, Atat1, facilitates the recruitment and processivity of molecular motors along MT tracks. However, the mechanisms that controls Atat1 activity remains poorly understood. Here we show that a pool of vesicular ATP-citrate lyase Acly acts as a rate limiting enzyme to modulate Atat1 activity by controlling availability of Acetyl-CoA. In addition, we showed that Acly expression is reduced upon loss of Elongator activity, further connecting Elongator to Atat1 in the pathway regulating -tubulin acetylation and MT-dependent transport in projection neurons across species. Remarkably, comparable defects occur in fibroblasts from Familial Dysautonomia (FD) patients bearing an autosomal recessive mutation in the gene coding for the Elongator subunit ELP1. Our data may thus shine new light on the pathophysiological mechanisms underlying FD.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_08:03:42.271.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-28 03:53:02	ID requested
⏵ 1	2022-02-16 08:03:43	announced

Even A, Morelli G, Turchetto S, Shilian M, Bail RL, Laguesse S, Krusy N, Brisker A, Brandis A, Inbar S, Chariot A, Saudou F, Dietrich P, Dragatsis I, Brone B, Broix L, Rigo JM, Weil M, Nguyen L, ATP-citrate lyase promotes axonal transport across species. Nat Commun, 12(1):5878(2021) [pubmed]

submitter keyword: transport, Citrate

Miguel Weil
contact affiliation	1Laboratory for Neurodegenerative Diseases and Personalized Medicine, School of Molecular Cell Biology and Biotechnology, The George S. Wise Faculty for Life Sciences, Sagol School of Neurosciences, Tel Aviv University, Ramat Aviv 69978, Israel
contact email	miguelw@tauex.tau.ac.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@tx.technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD021186

PRIDE project URI

• PRIDE
  1. PXD021186
     1. Label: PRIDE project
     2. Name: ATP-Citrate lyase fuels axonal transport across species