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PXD021180 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTMT analysis of LARP6 knockdown impact on protrusion induced changes in the proteome of MDA-MB231 cells
DescriptionTo assess if LARP6 is required for upregulation of ribosomal proteins upon protrusion induction, MDA-MB231 breast cancer cells were treated with non-targeting (NT) or LARP6 siRNA for 48 hrs, before being seeded on top of collagen-I coated 3 micron transwell filters (corning). No media was added to the bottom chamber and cells were allowed to adhere to filter overnight. The next day, the media on top of cells was refreshed, and the filters were either left with no media in the bottom chamber (closed pores), or with media also added to the bottom chamber (open pores) in order to allow formation of protrusions. Cells were allowed to form protrusions for 12 hrs before being lysed and analysed by mass spectrometry.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaria Dermit Salazar
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-27 23:48:10ID requested
12020-10-19 22:20:00announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: MDA-MB231
Contact List
Faraz Mardakheh
contact affiliationCentre for Cancer Cell & Molecular Biology, Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, The United Kingdom.
contact emailf.mardakheh@qmul.ac.uk
lab head
Maria Dermit Salazar
contact affiliationqmul
contact emailmariaprideproteomics@gmail.com
dataset submitter
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Dataset FTP location
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