PXD021179

PXD021179 is an original dataset announced via ProteomeXchange.

Title	Title- Modulation of implanted aortic scaffold- involvement of ECM and immune proteins
Description	Development of a suitable biological scaffold is an essential pre-requisite for functional development of organ including blood vessel. Biological scaffold prepared by conventional means using physical or chemical agent have been shown to cause damage to the extracellular matrix architecture as well as produce immune response subsequent to scaffold implantation/engraftment. During the current study, taking advantage of different host tissue environment (subcutaneous vs omentum), acellular scaffold was prepared using a syngeneic vs allogeneic model of scaffold implantation (in vivo decellularization) and compared with a chemically decellularized scaffold. By means of molecular, proteomics and histologic techniques, we confirmed that site specific advantages exist in modulating the ECM as well as regulating the immune response (macrophage and T-cells) to produce an acellular scaffold without the need for immunosuppressants. The current approach opens up the possibility for creation of tailor made scaffold for further use in the clinics with a possibility for long term acceptability without adverse reactions in the host and a further possibility to build functional organs following cell engraftment.
HostingRepository	PRIDE
AnnounceDate	2026-07-06
AnnouncementXML	Submission_2026-07-06_04:35:26.304.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carina Sihlbom
SpeciesList	scientific name: Rattus rattus (Black rat); NCBI TaxID: NEWT:10117; scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-27 23:44:52	ID requested
⏵ 1	2026-07-06 04:35:27	announced

Banerjee D, Nayakawde NB, Antony D, Deshmukh M, Ghosh S, Sihlbom C, Berger E, Ul Haq U, Olausson M, Characterization of Decellularized Implants for Extracellular Matrix Integrity and Immune Response Elicitation. Tissue Eng Part A, 28(13-14):621-639(2022) [pubmed]

10.1089/ten.tea.2021.0146;

submitter keyword: acellular, proteomics, immune, TMT, implant, macrophages,vascular scaffold, syn/allogeneic, extracellular matrix

Carina Sihlbom
contact affiliation	Proteomics Core Facility, University of Gothenburg
contact email	carina.sihlbom@gu.se
lab head
Carina Sihlbom
contact affiliation	Proteomics Core Facility / University of Gothenburg
contact email	carina.sihlbom@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD021179

PRIDE project URI

• PRIDE
  1. PXD021179
     1. Label: PRIDE project
     2. Name: Title- Modulation of implanted aortic scaffold- involvement of ECM and immune proteins