PXD021172

PXD021172 is an original dataset announced via ProteomeXchange.

Title	In vivo dynamics of Iron Starvation Induced Proteins under variable iron supplementation regimes suggest an iron processing organelle in Phaeodactylum tricornutum.
Description	The model pennate diatom Phaeodactylum tricornutum is able to assimilate a range of iron sources. It therefore provides a platform to study different mechanisms of iron processing concomitantly in the same cell. In this study we follow the localisation of three iron starvation induced proteins (ISIPs) in vivo, driven by their native promoters and tagged by fluorophores in an engineered line of P. tricornutum. In a proteomic analysis, we identify that the cell engages the endocytosis machinery involved in the vesicular trafficking as a response to siderophore molecules, even when these are not bound to iron. We find that the localisation patterns of ISIPs are dynamic and variable depending on the overall iron status of the cell and the source of iron it is exposed to. Notwithstanding, a common destination of the proteins is a globular compartment in the vicinity of the chloroplast, consistent with our proteomic findings, which point to the engagement of the cell’s vesicular network. We propose that this may be a dedicated iron processing site inside the periplastidial compartment of diatoms.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:46:30.259.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laurent Lignières
SpeciesList	scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850;
ModificationList	O4'-phospho-L-tyrosine; O-phospho-L-threonine; monohydroxylated residue; acetylated residue; O-phospho-L-serine; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-27 06:54:48	ID requested
1	2023-03-10 15:15:00	announced
⏵ 2	2023-11-14 07:46:32	announced	2023-11-14: Updated project metadata.

Kazamia E, Mach J, McQuaid JB, Gao X, Coale TH, Malych R, Camadro JM, Lesuisse E, Allen AE, Bowler C, Sutak R, . Plant Direct, 6(12):e472(2022) [pubmed]

submitter keyword: Iron Starvation Induced Proteins, fluorescent proteins,Diatoms, siderophores, iron, proteome, Phaeodactylum tricornutum

Jean-Michel Camadro
contact affiliation	Institut Jacques Monod - CNRS & Université de Paris
contact email	jean-michel.camadro@ijm.fr
lab head
Laurent Lignières
contact affiliation	Proteomic facility of Institut Jacques Monod (UMR7592) - CNRS & Université de Paris
contact email	laurent.lignieres@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021172
     1. Label: PRIDE project
     2. Name: In vivo dynamics of Iron Starvation Induced Proteins under variable iron supplementation regimes suggest an iron processing organelle in Phaeodactylum tricornutum.