PXD021163

PXD021163 is an original dataset announced via ProteomeXchange.

Title	PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus
Description	Type IVa pili are ubiquitous and versatile bacterial cell surface filaments that undergo cycles of extension, adhesion and retraction powered by the cell-envelope spanning type IVa pilus machine (T4aPM). The overall architecture of the T4aPM and the location of 10 conserved core proteins within this architecture have been elucidated. Here, using genetics, cell biology, proteomics and cryo-electron tomography, we demonstrate that the PilY1 protein and four minor pilins, which are widely conserved in T4aP systems, are essential for pilus extension in Myxococcus xanthus and form a complex that is an integral part of the T4aPM. Moreover, these proteins are part of the extended pilus. Our data support a model whereby the PilY1/minor pilin complex functions as a priming complex in T4aPM for pilus extension, a tip complex in the extended pilus for adhesion, and a cork for terminating retraction to maintain a priming complex for the next round of extension.
HostingRepository	PRIDE
AnnounceDate	2020-09-02
AnnouncementXML	Submission_2020-09-02_01:29:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021163
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Myxococcus xanthus DK 1622; NCBI TaxID: 246197;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-27 03:33:08	ID requested
⏵ 1	2020-09-02 01:29:39	announced

Dataset with its publication pending

submitter keyword: Type 4 Pil, shotgun proteomics, LFQi

Timo Glatter
contact affiliation	Max Planck Institute for terrestrial Microbiology Kar-von-Frisch Str. 10 35091 Marburg Germany
contact email	glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	MPI Marburg
contact email	glatter@mpi-marburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021163
     1. Label: PRIDE project
     2. Name: PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus