PXD021163 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus |
Description | Type IVa pili are ubiquitous and versatile bacterial cell surface filaments that undergo cycles of extension, adhesion and retraction powered by the cell-envelope spanning type IVa pilus machine (T4aPM). The overall architecture of the T4aPM and the location of 10 conserved core proteins within this architecture have been elucidated. Here, using genetics, cell biology, proteomics and cryo-electron tomography, we demonstrate that the PilY1 protein and four minor pilins, which are widely conserved in T4aP systems, are essential for pilus extension in Myxococcus xanthus and form a complex that is an integral part of the T4aPM. Moreover, these proteins are part of the extended pilus. Our data support a model whereby the PilY1/minor pilin complex functions as a priming complex in T4aPM for pilus extension, a tip complex in the extended pilus for adhesion, and a cork for terminating retraction to maintain a priming complex for the next round of extension. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-02 |
AnnouncementXML | Submission_2020-09-02_01:29:38.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021163 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Timo Glatter |
SpeciesList | scientific name: Myxococcus xanthus DK 1622; NCBI TaxID: 246197; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-27 03:33:08 | ID requested | |
⏵ 1 | 2020-09-02 01:29:39 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Type 4 Pil, shotgun proteomics, LFQi |
Contact List
Timo Glatter |
contact affiliation | Max Planck Institute for terrestrial Microbiology Kar-von-Frisch Str. 10 35091 Marburg Germany |
contact email | glatter@mpi-marburg.mpg.de |
lab head | |
Timo Glatter |
contact affiliation | MPI Marburg |
contact email | glatter@mpi-marburg.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021163
- Label: PRIDE project
- Name: PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus