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PXD021163

PXD021163 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus
DescriptionType IVa pili are ubiquitous and versatile bacterial cell surface filaments that undergo cycles of extension, adhesion and retraction powered by the cell-envelope spanning type IVa pilus machine (T4aPM). The overall architecture of the T4aPM and the location of 10 conserved core proteins within this architecture have been elucidated. Here, using genetics, cell biology, proteomics and cryo-electron tomography, we demonstrate that the PilY1 protein and four minor pilins, which are widely conserved in T4aP systems, are essential for pilus extension in Myxococcus xanthus and form a complex that is an integral part of the T4aPM. Moreover, these proteins are part of the extended pilus. Our data support a model whereby the PilY1/minor pilin complex functions as a priming complex in T4aPM for pilus extension, a tip complex in the extended pilus for adhesion, and a cork for terminating retraction to maintain a priming complex for the next round of extension.
HostingRepositoryPRIDE
AnnounceDate2020-09-02
AnnouncementXMLSubmission_2020-09-02_01:29:38.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD021163
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTimo Glatter
SpeciesList scientific name: Myxococcus xanthus DK 1622; NCBI TaxID: 246197;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-27 03:33:08ID requested
12020-09-02 01:29:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Type 4 Pil, shotgun proteomics, LFQi
Contact List
Timo Glatter
contact affiliationMax Planck Institute for terrestrial Microbiology Kar-von-Frisch Str. 10 35091 Marburg Germany
contact emailglatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliationMPI Marburg
contact emailglatter@mpi-marburg.mpg.de
dataset submitter
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Dataset FTP location
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