PXD021161

PXD021161 is an original dataset announced via ProteomeXchange.

Title	Identification of a sensor histidine kinase (BfcK) controlling biofilm formation in Clostridium acetobutylicum
Description	Clostridium acetobutylicum has been extensively exploited to produce biofuels and solvents and its biofilm could dramatically improve its productivities. However, genetic control of C. acetobutylicum biofilm has not been dissected so far. Here, a total of 24 disruptants were finally obtained over several years of attempts. Biofilm formation and physiological phenotypes were characterized for these disruptants and most of them showed robust biofilm formation still, or showed both impaired biofilm formation and cell growth. Only a mutant with a disputed histidine kinase gene (CA_C2730, designated bfcK in this study) abolished biofilm formation without impaired cell growth or solvent production. Phosphoproteomic analysis revealed that bfcK could control C. acetobutylicum flagellar motility at both translational and post-translational (protein phosphorylation) levels. The bfcK also showed apparent regulation of a serine/threonine protein kinase (encoded by CA_C0404) which was involved in protein secretion. Based on these findings, possible bfcK-based mechanisms for biofilm formation in C. acetobutylicum were proposed.
HostingRepository	PRIDE
AnnounceDate	2021-07-30
AnnouncementXML	Submission_2021-07-30_06:56:53.926.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong Liu
SpeciesList	scientific name: Clostridium acetobutylicum; NCBI TaxID: 1488;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-27 01:47:16	ID requested
⏵ 1	2021-07-30 06:56:54	announced

Dataset with its publication pending

submitter keyword: Clostridium acetobutylicum； biofilm；histidine kinases； phosphoproteomics；flagellar motility

Dong Liu
contact affiliation	College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
contact email	liudong@njtech.edu.cn
lab head
Dong Liu
contact affiliation	Nanjing Tech University
contact email	liudong@njtech.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021161
     1. Label: PRIDE project
     2. Name: Identification of a sensor histidine kinase (BfcK) controlling biofilm formation in Clostridium acetobutylicum