⮝ Full datasets listing

PXD021145

PXD021145 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleC4PR_LIV: N-terminomic analysis of SARS-CoV-2-infected ACE2-A549 cells (Unenriched)
DescriptionMany events in viral infection are regulated through post-translational modification. In the case of positive-sense RNA viruses such as SARS-CoV-2 which encodes two viral proteases, proteolytic cleavage of both viral and cellular proteins is crucial for productive viral infection. In this work we study proteolysis in the context of SARS-CoV-2 infection of ACE2-A549 and Vero E6 cells, infected at a multiplicity of infection of 1, with samples harvested at 0,6,12 and 24h post-infection. Neo-N-termini and lysine residues were labelled at the protein level with TMTpro reagents prior to tryptic digestion. For unenriched samples, the trypsin-digested material was then fractionated offline, and analysed by LC-MS/MS for the study of total protein abundance over the infection timecourse. For enriched samples, unblocked neo-N-termini generated by tryptic digested were labelled with undecanal and depleted, yielding a sample enriched for blocked neo-N-termini (TMTpro, N-terminal acetylation, and pyroGlu). The enriched samples were similarly fractionated offline and analysed by LC-MS/MS. Please note this is one of several linked datasets. This dataset contains data from ACE2-A549 cells infected with SARS-CoV-2, prior to enrichment.
HostingRepositoryPRIDE
AnnounceDate2021-07-29
AnnouncementXMLSubmission_2021-07-29_03:33:20.497.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEd Emmott
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-26 07:57:07ID requested
12021-07-29 03:33:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: C4PR_LIV: N-terminomic analysis of SARS-CoV-2-infected ACE2-A549 cells (Unenriched)
Contact List
Edward Emmott
contact affiliationCentre for Proteome Research, University of Liverpool, Liverpool, UK
contact emaile.emmott@liverpool.ac.uk
lab head
Ed Emmott
contact affiliationCentre for Proteome Research, University of Liverpool
contact emaile.emmott@liverpool.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD021145
PRIDE project URI
Repository Record List
[ + ]