PXD021143

PXD021143 is an original dataset announced via ProteomeXchange.

Title	Interactome of S65 phosphorylated NEDD8 and Ub
Description	In this project we present for the first time a proteome-wide interaction study of S65 phosphorylated NEDD8 and ubiquitin (Ub). By using cell extracts and affinity enrichment coupled to mass spectrometry, we show that pNEDD8 and pUb have different interactomes that are also distinct from those of the non-modified forms. Among the preferential pNEDD8 interactors are HSP70 family members and, indeed, pNEDD8 stimulates HSP70 ATPase activity more pronouncedly than non-modified NEDD8. Our findings provide evidence that phosphorylation of Ub and NEDD8 at S65 is involved in stress response signaling and underscore the importance of studying non-covalent interactions of NEDD8 in particular and post-translational modifications of Ubls in general.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_02:48:23.612.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katrin Stuber
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-26 07:44:28	ID requested
⏵ 1	2021-11-03 02:48:23	announced

Dataset with its publication pending

submitter keyword: NEDD8, ubiquitin, phosphorylation, interactome

Prof. Dr. Martin Scheffner
contact affiliation	Laboratory of Cellular Biochemistry, Department of Biology, University of Konstanz, Germany
contact email	Martin.Scheffner@uni-konstanz.de
lab head
Katrin Stuber
contact affiliation	Scheffner Group, Department of Biology, University of Konstanz
contact email	Katrin.Stuber@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD021143

PRIDE project URI

• PRIDE
  1. PXD021143
     1. Label: PRIDE project
     2. Name: Interactome of S65 phosphorylated NEDD8 and Ub