PXD021135

PXD021135 is an original dataset announced via ProteomeXchange.

Title	Activation of Wnt/β-catenin signaling in subchondral bone osteoblast responced to hypoxia lead to osteoarthritis-like phenotype in articular chondrocyte
Description	The crosstalk between the cartilage and subchondral bone plays a crucial role in the pathogenesis and progression of OA. The aim of this study was to identify soluble mediators released by osteoblasts that could induce the release of catabolic factors by chondrocytes. Global protein sequencing of subchondral bone samples isolated from OA patients was conducted by MS to discover potentially differently expressed protein. And the expression of candidate protein was validated by immunohistochemistry. The HCM or NCM human primary osteoblasts was used to stimulate human primary chondrocytes. Chondrocyte expression of type II collagen (COL2A1), aggrecan (ACAN), SOX9, MMP13 and MMP3 was assessed by RT-PCR. The soluble mediators released by hypoxia osteoblasts were indentified by RT-PCR, Western blotting, and Elisa. The serum concentrations of Wnt-related protein were further determined by Elisa. The proteomics and validated experiment revealed that procoagulation and hypoxia in the subchondral bone of OA. Stimulation of human primary chondrocytes with HCM significantly induced the mRNA expression of MMP13 and MMP3, and inhibited the mRNA of COL2A1, ACAN and SOX9. The identify of differential protein revealed that Wnt related protein (β-catenin) and Wnt antagonist (SOST) were up-regulated and down-regulated in hypoxia osteoblasts, separately. Furthermore, DKK-1 was significantly increased in human OA serum. The primary finding of this work was the identification that procoagulation and hypoxia in subchondral bone is the key factor of OA’s pathogenesis and progression, which facilitated chondrocyte phenotype shift towards Osteoarthritis-like, by activation Wnt/β-catenin signaling pathway in osteoblasts.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_07:39:01.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tengjiao Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-26 02:21:55	ID requested
⏵ 1	2025-05-06 07:39:02	announced

10.1021/acs.jproteome.3c00584;

submitter keyword: osteoarthritis(OA)

subchondral bone

articular cartilage

procoagulation

hypoixa

Wnt pathway

β-catenin;

Zhu Tengjiao
contact affiliation	Orthopedic Department, Peking University Third Hospital, Beijing 100191, P.R. China
contact email	zhutj@bjmu.edu.cn
lab head
Tengjiao Zhu
contact affiliation	Peking University Third Hospital
contact email	zhutj@bjmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021135
     1. Label: PRIDE project
     2. Name: Activation of Wnt/β-catenin signaling in subchondral bone osteoblast responced to hypoxia lead to osteoarthritis-like phenotype in articular chondrocyte