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PXD021135

PXD021135 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleActivation of Wnt/β-catenin signaling in subchondral bone osteoblast responced to hypoxia lead to osteoarthritis-like phenotype in articular chondrocyte
DescriptionThe crosstalk between the cartilage and subchondral bone plays a crucial role in the pathogenesis and progression of OA. The aim of this study was to identify soluble mediators released by osteoblasts that could induce the release of catabolic factors by chondrocytes. Global protein sequencing of subchondral bone samples isolated from OA patients was conducted by MS to discover potentially differently expressed protein. And the expression of candidate protein was validated by immunohistochemistry. The HCM or NCM human primary osteoblasts was used to stimulate human primary chondrocytes. Chondrocyte expression of type II collagen (COL2A1), aggrecan (ACAN), SOX9, MMP13 and MMP3 was assessed by RT-PCR. The soluble mediators released by hypoxia osteoblasts were indentified by RT-PCR, Western blotting, and Elisa. The serum concentrations of Wnt-related protein were further determined by Elisa. The proteomics and validated experiment revealed that procoagulation and hypoxia in the subchondral bone of OA. Stimulation of human primary chondrocytes with HCM significantly induced the mRNA expression of MMP13 and MMP3, and inhibited the mRNA of COL2A1, ACAN and SOX9. The identify of differential protein revealed that Wnt related protein (β-catenin) and Wnt antagonist (SOST) were up-regulated and down-regulated in hypoxia osteoblasts, separately. Furthermore, DKK-1 was significantly increased in human OA serum. The primary finding of this work was the identification that procoagulation and hypoxia in subchondral bone is the key factor of OA’s pathogenesis and progression, which facilitated chondrocyte phenotype shift towards Osteoarthritis-like, by activation Wnt/β-catenin signaling pathway in osteoblasts.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_07:39:01.495.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTengjiao Zhu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-26 02:21:55ID requested
12025-05-06 07:39:02announced
Publication List
10.1021/acs.jproteome.3c00584;
Tan Q, Li F, Zhang K, Liu Z, Tian Y, Zhu T, Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis. J Proteome Res, 23(2):738-748(2024) [pubmed]
Keyword List
submitter keyword: osteoarthritis(OA)
subchondral bone
articular cartilage
procoagulation
hypoixa
Wnt pathway
β-catenin;
Contact List
Zhu Tengjiao
contact affiliationOrthopedic Department, Peking University Third Hospital, Beijing 100191, P.R. China
contact emailzhutj@bjmu.edu.cn
lab head
Tengjiao Zhu
contact affiliationPeking University Third Hospital
contact emailzhutj@bjmu.edu.cn
dataset submitter
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Dataset FTP location
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