PXD021133

PXD021133 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of common allergenic tree pollens of birch, alder and hazelComparative proteomics of common allergenic tree pollens of birch, alder and hazel
Description	It is known that, in addition to known allergens, other proteins in pollen can aid the development of an immune response in allergenic individuals. The contribution of the “unknown” protein allergens becomes especially prominent in phylogenetically related species where, despite of high homology of the lead allergens, the degree of allergenic potential can greatly vary. The aim of this study was to identify other potentially allergenic proteins in pollen of three common and very related allergenic tree species: birch (Betula pendula), hazel (Corylus avellana) and alder (Alnus glutinosa). For that purpose, we carried out a comprehensive, comparative proteomic screening of the pollen from the three species. In order to maximize protein recovery and coverage, different protein extraction and isolation strategies during sample preparation were employed. As a result, we report 2500 - 3000 identified proteins per each of the pollen species. Identified proteins were further used for a number of annotation steps, providing insight into differential distribution of peptidases, peptidase inhibitors and other potential allergenic proteins across the three species. Moreover, we carried out functional enrichment analysis that, interestingly, corroborated high species similarity in spite of their relatively distinct protein profiles. We provide to our knowledge first insight into proteomes of three very important allergenic pollen types, which is particularly vital for pollen of hazel and alder where not even transcriptomics data is available. Datasets provided in this study can be readily used as protein databases, and as such serve as an excellent starting point for further allergenic studies.
HostingRepository	PRIDE
AnnounceDate	2021-04-21
AnnouncementXML	Submission_2021-04-20_22:57:23.036.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Darnhofer
SpeciesList	scientific name: Betula pendula; NCBI TaxID: 3505; scientific name: Alnus glutinosa; NCBI TaxID: 3517; scientific name: Corylus avellana; NCBI TaxID: 13451;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-26 02:21:39	ID requested
1	2021-04-20 21:52:24	announced
⏵ 2	2021-04-20 22:57:23	announced	2021-04-21: Updated project metadata.

Darnhofer B, Tomin T, Liesinger L, Schittmayer M, Tomazic PV, Birner-Gruenberger R, Comparative proteomics of common allergenic tree pollens of birch, alder, and hazel. Allergy, 76(6):1743-1753(2021) [pubmed]

10.1111/ALL.14694;

submitter keyword: birch, alder, proteomics,pollen, hazel

Ruth Birner-Gruenberger
contact affiliation	Full Professor for Analytical Chemistry Vienna University of Technology Institute of Chemical Technologies and Analytics Getreidemarkt 9/E164 A-1060 Vienna Austria
contact email	ruth.birner-gruenberger@tuwien.ac.at
lab head
Barbara Darnhofer
contact affiliation	Omics Center Graz
contact email	barbara.darnhofer@klinikum-graz.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021133
     1. Label: PRIDE project
     2. Name: Comparative proteomics of common allergenic tree pollens of birch, alder and hazelComparative proteomics of common allergenic tree pollens of birch, alder and hazel