PXD021123

PXD021123 is an original dataset announced via ProteomeXchange.

Title	NCAM1: A Novel Autoantigen in Membranous Lupus Nephritis
Description	Membranous lupus nephritis is a frequent cause of nephrotic syndrome in patients with systemic lupus erythematosus. Unlike phospholipase A2 receptor or thrombospondin type 1 domain containing 7A-associated membranous nephropathy, where known antibodies can be detected within sera by indirect immunofluorescence and/or enzyme-linked immunosorbent assay, it is not possible to monitor disease activity in membranous lupus nephritis where the target autoantigens are mostly unknown. Determination of the target autoantigen has diagnostic significance, informs prognosis, and allows for non-invasive monitoring of disease activity in serum. We utilized mass spectrometry for antigen discovery of laser capture microdissected glomeruli from formalin-fixed paraffin embedded tissue and tissue IgG immunoprecipitation studies from frozen kidney biopsy tissue. We identified neural cell adhesion molecule 1 (NCAM1) to be a target antigen in membranous lupus nephritis and within rare cases of primary membranous nephropathy. The prevalence of NCAM1-associated membranous neuropathy was 5.7% of cases of membranous lupus nephritis. NCAM1 co-localizes with IgG within glomerular immune deposits. Additionally, serum from NCAM1 patients showed reactivity to NCAM1 recombinant protein. The presence of anti-NCAM1 antibodies in sera could allow for non-invasive monitoring of the disease. We propose that NCAM1 is a target autoantigen in a subset of patients with membranous lupus nephritis. Future studies are needed to determine whether anti-NCAM1 antibody levels correlate with disease activity or response to therapy.
HostingRepository	PRIDE
AnnounceDate	2020-10-19
AnnouncementXML	Submission_2020-10-19_01:09:45.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021123
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Aaron Storey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-26 00:01:04	ID requested
⏵ 1	2020-10-19 01:09:46	announced

Caza TN, Hassen SI, Kuperman M, Sharma SG, Dvanajscak Z, Arthur J, Edmondson R, Storey A, Herzog C, Kenan DJ, Larsen CP, Neural cell adhesion molecule 1 is a novel autoantigen in membranous lupus nephritis. Kidney Int, 100(1):171-181(2021) [pubmed]

submitter keyword: Human, Kidney, DDA

Chris Larsen
contact affiliation	Arkana Laboratories, Little Rock, Arkansas, USA
contact email	chris.larsen@arkanalabs.com
lab head
Aaron Storey
contact affiliation	University of Arkansas for Medical Sciences
contact email	ajstorey@uams.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021123
     1. Label: PRIDE project
     2. Name: NCAM1: A Novel Autoantigen in Membranous Lupus Nephritis