PXD021115 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HspB8 controls molecular aging of FUS condensates by domain-specific protein interactions Part 2 |
Description | Biomolecular condensates formed by phase separation offer a confined space where protein-protein interactions (PPIs) facilitate distinct biochemical reactions. As their aberrant behavior is associated with disease, it is crucial to understand the molecular organization of PPIs within condensates. Using quantitative time-resolved crosslinking mass spectrometry (XL-MS) we directly and specifically monitor PPIs and protein dynamics inside condensates formed by the protein fused in sarcoma (FUS) and identify its folded RNA recognition motif (RRM) as a key player of aberrant molecular aging. We find that the chaperone HspB8, but not a disease-associated mutant, prevents FUS aging. In XL-MS and partitioning experiments we find that the disordered region of HspB8 directs its α-crystallin domain (αCD) into FUS droplets where HspB8 prevents aging via condensate-specific αCD–RRM interactions. We propose that chaperones like HspB8 prevent aberrant phase transitions by stabilizing aggregation prone folded domains inside condensates in times of cellular stress. |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-13 |
AnnouncementXML | Submission_2021-10-12_23:15:20.791.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Florian Stengel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-25 09:25:39 | ID requested | |
⏵ 1 | 2021-10-12 23:15:21 | announced | |
Publication List
Boczek EE, F, ü, rsch J, Niedermeier ML, Jawerth L, Jahnel M, Ruer-Gru, ß M, Kammer KM, Heid P, Mediani L, Wang J, Yan X, Pozniakovski A, Poser I, Mateju D, Hubatsch L, Carra S, Alberti S, Hyman AA, Stengel F, HspB8 prevents aberrant phase transitions of FUS by chaperoning its folded RNA-binding domain. Elife, 10():(2021) [pubmed] |
Keyword List
submitter keyword: FUS, aging, chaperone, structural proteomics, time-resolved quantitative XL-MS, RRM, protein dynamics, molecular condensates |
Contact List
Florian Stengel |
contact affiliation | University of Konstanz;Biology |
contact email | florian.stengel@uni-konstanz.de |
lab head | |
Florian Stengel |
contact affiliation | Universität Konstanz |
contact email | florian.stengel@uni-konstanz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021115
- Label: PRIDE project
- Name: HspB8 controls molecular aging of FUS condensates by domain-specific protein interactions Part 2