PXD021107

PXD021107 is an original dataset announced via ProteomeXchange.

Title	Stage-specific protein regulation during somatic embryo development of Carica papaya L. ‘Golden’
Description	Somatic embryogenesis is an important biotechnological technique for large-scale propagation of elite genotypes. Correlations of stage-specific compounds associated with somatic embryo development can help elucidate the ontogenesis of Carica papaya L. somatic embryos and improve tissue culture protocols. To identify the stage-specific proteins that are present during the differentiation of C. papaya somatic embryos, proteomic analyses of embryos at the globular, heart, torpedo and cotyledonary stages were performed. Comparative proteomic analysis revealed a total of 801 proteins, 392 of which were classified as differentially accumulated proteins in at least one of the developmental stages. The globular stage presented a higher number of unique proteins (16), 7 of which were isoforms of 60S ribosomal proteins, suggesting high translational activity at the beginning of somatic embryogenesis. Proteins related to mitochondrial metabolism accumulated to a high degree at the early developmental stages, after which they decreased with increasing development, contributing to cell homeostasis in early somatic embryos. On the other hand, a progressive increase in the accumulation of vicilin, late embryogenesis abundant proteins and chloroplastic proteins leading to somatic embryo maturation was observed. Additionally, the differential accumulation of acetylornithine deacetylase and S-adenosylmethionine synthase 2 proteins correlated with increased contents of putrescine and spermidine, suggesting that polyamine metabolism is important to somatic embryo development. Taken together, the results showed that somatic embryo development in C. papaya is regulated by the differential accumulation of proteins, with ribosomal and mitochondrial proteins being more abundant during the early somatic embryo stages, while proteins involved in seed maturation are more abundant during the late stages.
HostingRepository	PRIDE
AnnounceDate	2020-11-13
AnnouncementXML	Submission_2020-11-13_07:19:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanildo Silveira
SpeciesList	scientific name: Carica papaya; NCBI TaxID: 3649;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-25 04:57:20	ID requested
⏵ 1	2020-11-13 07:19:19	announced

Botini N, Almeida FA, Cruz KZCM, Reis RS, Vale EM, Garcia AB, Santa-Catarina C, Silveira V, Stage-specific protein regulation during somatic embryo development of Carica papaya L. 'Golden'. Biochim Biophys Acta Proteins Proteom, 1869(2):140561(2021) [pubmed]

submitter keyword: Somatic embryogenesis, C. papaya, Label-free proteomics, Polyamines

Vanildo Silveira
contact affiliation	Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia Universidade Estadual do Norte Fluminense Darcy Ribeiro Av. Alberto Lamego 2000 Campos dos Goytacazes-RJ, Brazil 28013-602
contact email	vanildo@gmail.com
lab head
Vanildo Silveira
contact affiliation	UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE
contact email	vanildo@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD021107

PRIDE project URI

• PRIDE
  1. PXD021107
     1. Label: PRIDE project
     2. Name: Stage-specific protein regulation during somatic embryo development of Carica papaya L. ‘Golden’