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PXD021102

PXD021102 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleiTRAQ analysis of ClpP mutant treated with methyl gallate
DescriptionMethyl gallate (MG) is an effective microbicide with great potential usefulness in the integrated management of plant diseases and an important potential drug for clinical application. However, its target remains unknown. This study conducted a transposon sequencing (Tn-seq) under MG treatment in plant pathogenic bacterium Ralstonia solanacearum. Tn-seq identified that the mutation of caseinolytic protease proteolytic subunit gene clpP significantly increased the resistance of R. solanacearum to MG, which was validated by the in-frame gene deletion. iTRAQ proteomics analysis was then employed and revealed that chemotaxis and flagella associated proteins were the major substrates degraded by ClpP under the tested condition. Moreover, sulfur metabolism associated proteins were also possible substrates of ClpP and were up-regulated by MG treatment in the wild type R. solanacearum but not in the clpP mutant. Furthermore, molecular docking confirmed the possible interaction between MG and ClpP. Taken together, this study revealed that MG may target bacterial ClpP, inhibit the activity of ClpP, and consequently disorder bacterial proteostasis, providing a theoretical basis for the application of MG.
HostingRepositoryPRIDE
AnnounceDate2020-12-10
AnnouncementXMLSubmission_2020-12-09_23:23:02.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDehong Zheng
SpeciesList scientific name: Ralstonia solanacearum GMI1000; NCBI TaxID: 267608;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-25 01:03:07ID requested
12020-12-09 23:23:03announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ClpP protease, methyl gallate, Ralstonia solanacearum
Contact List
dehong Zheng
contact affiliationGuangxi University
contact emaildehong@gxu.edu.cn
lab head
Dehong Zheng
contact affiliationGuangxi University
contact emaildehong@gxu.edu.cn
dataset submitter
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