PXD021102 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | iTRAQ analysis of ClpP mutant treated with methyl gallate |
Description | Methyl gallate (MG) is an effective microbicide with great potential usefulness in the integrated management of plant diseases and an important potential drug for clinical application. However, its target remains unknown. This study conducted a transposon sequencing (Tn-seq) under MG treatment in plant pathogenic bacterium Ralstonia solanacearum. Tn-seq identified that the mutation of caseinolytic protease proteolytic subunit gene clpP significantly increased the resistance of R. solanacearum to MG, which was validated by the in-frame gene deletion. iTRAQ proteomics analysis was then employed and revealed that chemotaxis and flagella associated proteins were the major substrates degraded by ClpP under the tested condition. Moreover, sulfur metabolism associated proteins were also possible substrates of ClpP and were up-regulated by MG treatment in the wild type R. solanacearum but not in the clpP mutant. Furthermore, molecular docking confirmed the possible interaction between MG and ClpP. Taken together, this study revealed that MG may target bacterial ClpP, inhibit the activity of ClpP, and consequently disorder bacterial proteostasis, providing a theoretical basis for the application of MG. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-10 |
AnnouncementXML | Submission_2020-12-09_23:23:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dehong Zheng |
SpeciesList | scientific name: Ralstonia solanacearum GMI1000; NCBI TaxID: 267608; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-25 01:03:07 | ID requested | |
⏵ 1 | 2020-12-09 23:23:03 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: ClpP protease, methyl gallate, Ralstonia solanacearum |
Contact List
dehong Zheng |
contact affiliation | Guangxi University |
contact email | dehong@gxu.edu.cn |
lab head | |
Dehong Zheng |
contact affiliation | Guangxi University |
contact email | dehong@gxu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021102
- Label: PRIDE project
- Name: iTRAQ analysis of ClpP mutant treated with methyl gallate