PXD021102

PXD021102 is an original dataset announced via ProteomeXchange.

Title	iTRAQ analysis of ClpP mutant treated with methyl gallate
Description	Methyl gallate (MG) is an effective microbicide with great potential usefulness in the integrated management of plant diseases and an important potential drug for clinical application. However, its target remains unknown. This study conducted a transposon sequencing (Tn-seq) under MG treatment in plant pathogenic bacterium Ralstonia solanacearum. Tn-seq identified that the mutation of caseinolytic protease proteolytic subunit gene clpP significantly increased the resistance of R. solanacearum to MG, which was validated by the in-frame gene deletion. iTRAQ proteomics analysis was then employed and revealed that chemotaxis and flagella associated proteins were the major substrates degraded by ClpP under the tested condition. Moreover, sulfur metabolism associated proteins were also possible substrates of ClpP and were up-regulated by MG treatment in the wild type R. solanacearum but not in the clpP mutant. Furthermore, molecular docking confirmed the possible interaction between MG and ClpP. Taken together, this study revealed that MG may target bacterial ClpP, inhibit the activity of ClpP, and consequently disorder bacterial proteostasis, providing a theoretical basis for the application of MG.
HostingRepository	PRIDE
AnnounceDate	2020-12-10
AnnouncementXML	Submission_2020-12-09_23:23:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dehong Zheng
SpeciesList	scientific name: Ralstonia solanacearum GMI1000; NCBI TaxID: 267608;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-25 01:03:07	ID requested
⏵ 1	2020-12-09 23:23:03	announced

Dataset with its publication pending

submitter keyword: ClpP protease, methyl gallate, Ralstonia solanacearum

dehong Zheng
contact affiliation	Guangxi University
contact email	dehong@gxu.edu.cn
lab head
Dehong Zheng
contact affiliation	Guangxi University
contact email	dehong@gxu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021102
     1. Label: PRIDE project
     2. Name: iTRAQ analysis of ClpP mutant treated with methyl gallate