PXD021101

PXD021101 is an original dataset announced via ProteomeXchange.

Title	The alterations of proteins expression related to insulin signaling pathway in the brain of phenobarbital addictive rats by TMT
Description	Drug addiction is a chronic relapsing brain disease. The alterations of glucose uptake and metabolism were found in the brain of drug addicts. Insulin mediates brain glucose metabolism and its abnormality could induce brain injury and cognitive impairment. Here, we established a rat model of phenobarbital addiction by 90 days dosed escalation and evaluated addiction-related symptoms. We also performed proteomic analysis of the function of the differentially expressed (DE) proteins via bioinformatics in brain tissues by LC-MS/MS on day 60 and 90 of phenobarbital or 0,5% CMC-Na (vehicle) administration. The results showed that phenobarbital addictive rats developed severe withdrawal symptoms after abstinence. Proteomics analysis showed that numerous DE proteins were enriched after phenobarbital administration; which CALM1, ARAF and Cbl proteins (related to insulin signaling pathway) were significantly downregulated on day 60 but no day 90; however, SLC27A3 and NF-kB1 proteins (related to insulin resistance) were significantly upregulated on day 90. Our data indicate that insulin signaling pathway and insulin resistance may play a role in the development of phenobarbital addiction and brain injury and the findings may have important clinical implication.
HostingRepository	PRIDE
AnnounceDate	2020-10-29
AnnouncementXML	Submission_2020-10-29_01:19:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guojun Wang
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-25 01:02:50	ID requested
⏵ 1	2020-10-29 01:19:19	announced

Wang M, Pu X, Feng B, Fan Q, Dai Y, Chen Y, Li Y, Liu L, Cao S, Wang G, F-FDG PET/CT and Proteomic Analysis. J Proteome Res, 20(1):950-959(2021) [pubmed]

submitter keyword: Addiction, Phenobarbital, Proteomics, Insulin signaling pathway, TMT

Guojun Wang
contact affiliation	Department of Pharmacy, The Affiliated Hospital of Southwest Medical University, China
contact email	renren333@126.com
lab head
Guojun Wang
contact affiliation	Department of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou. Sichuan 646000, China
contact email	renren333@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021101
     1. Label: PRIDE project
     2. Name: The alterations of proteins expression related to insulin signaling pathway in the brain of phenobarbital addictive rats by TMT