PXD021098

PXD021098 is an original dataset announced via ProteomeXchange.

Title	Fecal proteomics as a novel method to study mammalian behavior and physiology
Description	Mammalian feces can be collected non-invasively during field research and provides valuable information on the ecology and evolution of the host individuals. Undigested food objects, genome/metagenome, steroid hormones, and stable isotopes obtained from fecal samples provide evidence on diet, host/symbiont genetics, and physiological status of the individuals. However, proteins in mammalian feces have hardly been studied, which hampers the molecular investigations into the behavior and physiology of the host individuals. Here, we apply mass spectrometry-based proteomics to fecal samples (n = 10) that were collected from infant, juvenile, and adult captive Japanese macaques (Macaca fuscata) to describe the proteomes of the host, food, and intestinal microbes. The results show that fecal proteomics is a useful method to investigate dietary changes along with breastfeeding and weaning, to reveal the organ/tissue and taxonomy of dietary items, and to estimate physiological status inside intestinal tracts. These types of insights are difficult or impossible to obtain through other molecular approaches. Most mammalian species are facing extinction risk and there is an urgent need to obtain knowledge on their ecology and evolution for better conservation strategy. The fecal proteomics framework we present here is easily applicable to wild settings and other mammalian species, and provides direct evidence of their behavior and physiology.
HostingRepository	PRIDE
AnnounceDate	2021-03-18
AnnouncementXML	Submission_2021-03-18_00:13:33.432.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Takumi Tsutaya
SpeciesList	scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-25 00:00:15	ID requested
1	2021-03-17 22:13:58	announced
⏵ 2	2021-03-18 00:13:34	announced	2021-03-18: Updated project metadata.

10.1111/1755-0998.13380;

Tsutaya T, Mackie M, Sawafuji R, Miyabe-Nishiwaki T, Olsen JV, Cappellini E, Faecal proteomics as a novel method to study mammalian behaviour and physiology. Mol Ecol Resour, 21(6):1808-1819(2021) [pubmed]

submitter keyword: Weaning, Feces, Diet, Japanese macaque,Breastfeeding, LC-MS/MS

Enrico Cappellini
contact affiliation	Section for Evolutionary Genomics, The Globe Institute, University of Copenhagen, Denmark
contact email	ecappellini@sund.ku.dk
lab head
Takumi Tsutaya
contact affiliation	The Graduate University for Advanced Studies
contact email	tsuta16@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021098
     1. Label: PRIDE project
     2. Name: Fecal proteomics as a novel method to study mammalian behavior and physiology