PXD021097

PXD021097 is an original dataset announced via ProteomeXchange.

Title	The Ubiquitin-modified Proteome of SARS-CoV-2-infected Host Cells Reveals Insights into Virus-Host Interaction and Pathogenesis
Description	The outbreak of coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has posed a serious threat to global public health. The mechanism of pathogenesis and host immune response to SARS-CoV-2 infection are largely unknown. In the present study, we applied a quantitative proteomic technology to identify and quantify the ubiquitination changes that occur in both the virus and Vero cells during SARS-CoV-2 infection. By applying label-free, quantitative LC-MS/MS proteomics, 8,943 lysine ubiquitination sites on 3,086 proteins were identified, of which 138 sites on 104 proteins were quantified as significantly upregulated, while 828 sites on 447 proteins were downregulated at 72 h post-infection. Bioinformatics analysis suggested that SARS-CoV-2 infection might modulate host immune responses through the ubiquitination of important proteins, including USP5, IQGAP1, TRIM28, and Hsp90. Ubiquitination modification was also observed on 11 SAR-CoV-2 proteins, including proteins involved in virus replication and inhibition of the host innate immune response. Our study provides new insights into the interaction between SARS-CoV-2 and the host as well as potential targets for the prevention and treatment of COVID-19.
HostingRepository	PRIDE
AnnounceDate	2021-03-08
AnnouncementXML	Submission_2021-03-07_22:33:56.299.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinying Zhu
SpeciesList	scientific name: SARS coronavirus GD322; NCBI TaxID: 291613;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-24 23:50:45	ID requested
⏵ 1	2021-03-07 22:33:57	announced

Zhang H, Zheng H, Zhu J, Dong Q, Wang J, Fan H, Chen Y, Zhang X, Han X, Li Q, Lu J, Tong Y, Chen Z, Ubiquitin-Modified Proteome of SARS-CoV-2-Infected Host Cells Reveals Insights into Virus-Host Interaction and Pathogenesis. J Proteome Res, 20(5):2224-2239(2021) [pubmed]

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: coronavirus disease 2019

severe acute respiratory syndrome coronavirus 2

LC-MS

ubiquitome

Zeliang Chen
contact affiliation	Key Laboratory of Zoonotic of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Liaoning Province, Shenyang 110866, P. R. China Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, 511430, P. R. China Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology,100029, P. R. China
contact email	zeliangchen@yahoo.com
lab head
Jinying Zhu
contact affiliation	Shenyang Agricultural University
contact email	jean_zz512@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD021097

PRIDE project URI

• PRIDE
  1. PXD021097
     1. Label: PRIDE project
     2. Name: The Ubiquitin-modified Proteome of SARS-CoV-2-infected Host Cells Reveals Insights into Virus-Host Interaction and Pathogenesis