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PXD021094

PXD021094 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic profiles of control, AUF1-overexpressed, and CVB3-infected HeLa cells
DescriptionCVB 3C protease (3Cpro) plays a specific cleavage role on AU-rich binding factor (AUF1, also called as hnRNP D), consequently disputes the regulations of AUF1 on downstream molecules stabilities and transcriptions. In our work, iTRAQ approach was firstly used to quantitatively identify differentially expressed cellular proteins in AUF1-overexpressed HeLa cells, which would provide us a new insight into the role of AUF1 on cellular progress. We identified 1290 differentially expressed proteins (DEPs), including 882 upregulated and 408 downregulated DEPs at 24 h post infection (p.i.), The DEPs are involved in a variety of cellular functions such as metabolic processes, transcription, and RNA binding and exhibit different subcellular localization via GO term, protein interaction network, and series analysis. Among DEPs, DDX5, POLR2, and PPP2CA showed important roles in cellular metabolism. Especially, DDX5 was negatively regulated by AUF1 and increased during CVB infection, which in turns played an inhibitory effect on CVB replication. These findings are particularly important in the development new anti-viral therapy strategies targeting on series of new molecules.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:25:35.447.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterwu jingqi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-24 23:06:08ID requested
12023-03-10 16:19:13announced
22023-11-14 07:25:40announced2023-11-14: Updated project metadata.
Publication List
Wang T, Qin Y, Chen J, Chen S, Wu J, Chen X, Chen X, Zhou S, Zou J, Guan J, Cleavage of AUF1 by Coxsackievirus B Affects DDX5 Regulatory on Viral Replication through iTRAQ Proteomics Analysis. Biomed Res Int, 2022():8610467(2022) [pubmed]
Keyword List
submitter keyword: LC-MS, lysate,Human HeLa cells, AUF1
Contact List
Tianying Wang
contact affiliationqingdao municipal hospital clinical research center
contact emailwangty0929@163.com
lab head
wu jingqi
contact affiliationHarbin medical university
contact email18846753646@163.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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