PXD021093

PXD021093 is an original dataset announced via ProteomeXchange.

Title	pre-mRNA splicing is regulated by an Aurora-A-mediated phopsho-signaling network
Description	In this study, we identified many new potential interactors of the Aurora-A kinase using a proteomic approach. A significant portion of Aurora-A interaction network is composed of proteins involved in pre-mRNA splicing, implying that Aurora-A signaling extends beyond its canonical function. Aurora-A directly interacts with many of the RRM domain-containing splicing factors such as SR proteins and hnRNP proteins and phosphorylates them in vitro. Aurora-A shows a subcellar distribution to nuclear speckles, the storehouse of splicing factors, consistent with its potential function in pre-mRNA splicing. Moreover, RNA-seq analysis of pharmacologically inhibited Aurora-A cells identified 261 genes whose RNA splicing is dependent on Aurora-A activity. These splicing affected genes are involved in various biological processes such as transcription, GTPase activity, ciliogenesis, DNA repair, RNA splicing and G2/M transition. Here, for the first time, we uncovered a relationship between Aurora-A activity and mRNA processing through a complex network of factors involved in RNA maturation.
HostingRepository	PRIDE
AnnounceDate	2024-12-11
AnnouncementXML	Submission_2024-12-11_06:36:14.998.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jean-Philippe Gagne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-24 23:03:29	ID requested
⏵ 1	2024-12-11 06:36:15	announced

Damodaran AP, Gavard O, Gagn, é JP, Rogalska ME, Behera AK, Mancini E, Bertolin G, Courtheoux T, Kumari B, Cailloce J, Mereau A, Poirier GG, Valc, á, rcel J, Gonatopoulos-Pournatzis T, Watrin E, Prigent C, Proteomic study identifies Aurora-A-mediated regulation of alternative splicing through multiple splicing factors. J Biol Chem, 301(1):108000(2025) [pubmed]

10.1016/j.jbc.2024.108000;

submitter keyword: aurora kinase,LC-MS/MS

Claude Prigent
contact affiliation	Université de Rennes-1, CNRS, Institut de Génétique et Développement de Rennes (IGDR) UMR6290, Equipe labelisée LNCC 2014, Rennes, France.
contact email	claude.prigent@univ-rennes1.fr
lab head
Jean-Philippe Gagne
contact affiliation	Laval University Medical Research Center
contact email	jean-philippe.gagne@crchudequebec.ulaval.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD021093

PRIDE project URI

• PRIDE
  1. PXD021093
     1. Label: PRIDE project
     2. Name: pre-mRNA splicing is regulated by an Aurora-A-mediated phopsho-signaling network