PXD021081 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Short-term mild temperature-stress-induced alterations in C. elegans phosphoproteome |
Description | Exposure to mild early-life stresses can slow down the aging process, in which protein phosphorylation might be an essential regulator. Currently, the understanding of phosphorylation-based regulatory networks under mild early-life stress remains elusive. Herein, we systematically analyzed the phosphoproteomes of C. elegans, which were treated with three kinds of mild temperature (15°C, 20°C, and 25°C) from two different short-term groups (10 min and 60 min). By utilizing iTRAQ-based quantitative phosphoproteomic approach, a total of 18187 phosphorylation sites from 3330 proteins were identified. Volcano plots illustrated that the phosphorylation abundance of 374 proteins (15°C) and 347 proteins (25°C), were significantly changed, respectively. Gene ontology, KEGG pathway and protein-protein interaction network analysis revealed that these phosphoproteins were mainly involved in metabolism, translation, development, and lifespan determination. Motif analysis of kinase substrates suggested that MAPK, CK and CAMK were most likely involved in the adaption processes. Moreover, 16 and 14 aging-regulated proteins underwent phosphorylation modifications under the mild stress of 15°C and 25°C, respectively, indicating these proteins might be important for maintaining long-term health. Further experiments of lifespan confirmed that the candidate phosphoproteins, e.g.; EGL-27, XNP-1, and GTBP-1 could regulate lifespan at 15°C, 20°C, and 25°C, and showed increased tolerance to heat and oxidative stresses. In summary, our findings provided a wealth of datasets to better understand the phosphorylation mechanism of mild early-life stresses in C. elegans. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-17 |
AnnouncementXML | Submission_2020-09-17_02:56:50.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jichang Huang |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-24 07:10:47 | ID requested | |
⏵ 1 | 2020-09-17 02:56:51 | announced | |
Publication List
Huang J, Wu Z, Zhang X, Phosphoproteome. Int J Mol Sci, 21(17):(2020) [pubmed] |
Keyword List
submitter keyword: Phosphoproteomics |
C. elegans |
mild temperature |
lifespan |
mild stresses |
Contact List
Xumin Zhang |
contact affiliation | State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University |
contact email | xumin_zhang@fudan.edu.cn |
lab head | |
Jichang Huang |
contact affiliation | School of Life Sciences, Fudan University |
contact email | 14110700074@fudan.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021081
- Label: PRIDE project
- Name: Short-term mild temperature-stress-induced alterations in C. elegans phosphoproteome