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PXD021081

PXD021081 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleShort-term mild temperature-stress-induced alterations in C. elegans phosphoproteome
DescriptionExposure to mild early-life stresses can slow down the aging process, in which protein phosphorylation might be an essential regulator. Currently, the understanding of phosphorylation-based regulatory networks under mild early-life stress remains elusive. Herein, we systematically analyzed the phosphoproteomes of C. elegans, which were treated with three kinds of mild temperature (15°C, 20°C, and 25°C) from two different short-term groups (10 min and 60 min). By utilizing iTRAQ-based quantitative phosphoproteomic approach, a total of 18187 phosphorylation sites from 3330 proteins were identified. Volcano plots illustrated that the phosphorylation abundance of 374 proteins (15°C) and 347 proteins (25°C), were significantly changed, respectively. Gene ontology, KEGG pathway and protein-protein interaction network analysis revealed that these phosphoproteins were mainly involved in metabolism, translation, development, and lifespan determination. Motif analysis of kinase substrates suggested that MAPK, CK and CAMK were most likely involved in the adaption processes. Moreover, 16 and 14 aging-regulated proteins underwent phosphorylation modifications under the mild stress of 15°C and 25°C, respectively, indicating these proteins might be important for maintaining long-term health. Further experiments of lifespan confirmed that the candidate phosphoproteins, e.g.; EGL-27, XNP-1, and GTBP-1 could regulate lifespan at 15°C, 20°C, and 25°C, and showed increased tolerance to heat and oxidative stresses. In summary, our findings provided a wealth of datasets to better understand the phosphorylation mechanism of mild early-life stresses in C. elegans.
HostingRepositoryPRIDE
AnnounceDate2020-09-17
AnnouncementXMLSubmission_2020-09-17_02:56:50.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJichang Huang
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-24 07:10:47ID requested
12020-09-17 02:56:51announced
Publication List
Huang J, Wu Z, Zhang X, Phosphoproteome. Int J Mol Sci, 21(17):(2020) [pubmed]
Keyword List
submitter keyword: Phosphoproteomics
C. elegans
mild temperature
lifespan
mild stresses
Contact List
Xumin Zhang
contact affiliationState Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University
contact emailxumin_zhang@fudan.edu.cn
lab head
Jichang Huang
contact affiliationSchool of Life Sciences, Fudan University
contact email14110700074@fudan.edu.cn
dataset submitter
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Dataset FTP location
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