PXD021081

PXD021081 is an original dataset announced via ProteomeXchange.

Title	Short-term mild temperature-stress-induced alterations in C. elegans phosphoproteome
Description	Exposure to mild early-life stresses can slow down the aging process, in which protein phosphorylation might be an essential regulator. Currently, the understanding of phosphorylation-based regulatory networks under mild early-life stress remains elusive. Herein, we systematically analyzed the phosphoproteomes of C. elegans, which were treated with three kinds of mild temperature (15°C, 20°C, and 25°C) from two different short-term groups (10 min and 60 min). By utilizing iTRAQ-based quantitative phosphoproteomic approach, a total of 18187 phosphorylation sites from 3330 proteins were identified. Volcano plots illustrated that the phosphorylation abundance of 374 proteins (15°C) and 347 proteins (25°C), were significantly changed, respectively. Gene ontology, KEGG pathway and protein-protein interaction network analysis revealed that these phosphoproteins were mainly involved in metabolism, translation, development, and lifespan determination. Motif analysis of kinase substrates suggested that MAPK, CK and CAMK were most likely involved in the adaption processes. Moreover, 16 and 14 aging-regulated proteins underwent phosphorylation modifications under the mild stress of 15°C and 25°C, respectively, indicating these proteins might be important for maintaining long-term health. Further experiments of lifespan confirmed that the candidate phosphoproteins, e.g.; EGL-27, XNP-1, and GTBP-1 could regulate lifespan at 15°C, 20°C, and 25°C, and showed increased tolerance to heat and oxidative stresses. In summary, our findings provided a wealth of datasets to better understand the phosphorylation mechanism of mild early-life stresses in C. elegans.
HostingRepository	PRIDE
AnnounceDate	2020-09-17
AnnouncementXML	Submission_2020-09-17_02:56:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jichang Huang
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-24 07:10:47	ID requested
⏵ 1	2020-09-17 02:56:51	announced

Huang J, Wu Z, Zhang X, Phosphoproteome. Int J Mol Sci, 21(17):(2020) [pubmed]

submitter keyword: Phosphoproteomics

C. elegans

mild temperature

lifespan

mild stresses

Xumin Zhang
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University
contact email	xumin_zhang@fudan.edu.cn
lab head
Jichang Huang
contact affiliation	School of Life Sciences, Fudan University
contact email	14110700074@fudan.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021081
     1. Label: PRIDE project
     2. Name: Short-term mild temperature-stress-induced alterations in C. elegans phosphoproteome