PXD021072

PXD021072 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Bridgin connects the outer kinetochore to centromeric chromatin
Description	In this project, 3XFlag-tagged protein affinity purifications were performed to identify the protein-protein interactors using Cryptococcus neoformans cell lysate. After fractionation with SDS-PAGE, peptide samples for nanoLC-MS/MS were prepared by in-gel digestion. Raw data from Orbotrap Velos pro were processed with MASCOT via Proteome discoverer2.2. Validation of peptide and protein identifications were performed with Scaffold proteome software.
HostingRepository	jPOST
AnnounceDate	2020-09-23
AnnouncementXML	Submission_2022-09-18_03:45:06.830.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021072
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Reiko Nakagawa
SpeciesList	scientific name: Cryptococcus neoformans; NCBI TaxID: 5207;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-24 00:54:19	ID requested
1	2020-09-23 01:09:18	announced
2	2021-01-10 02:47:38	announced	2021-01-10: Updated PubMed.
3	2021-02-04 17:23:07	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 03:45:07	announced	2022-09-18: Updated FTP location.

Publication List

Sridhar S, Hori T, Nakagawa R, Fukagawa T, Sanyal K, Bridgin connects the outer kinetochore to centromeric chromatin. Nat Commun, 12(1):146(2021) [pubmed]

Sridhar S, Hori T, Nakagawa R, Fukagawa T, Sanyal K, Bridgin connects the outer kinetochore to centromeric chromatin. Nat Commun, 12(1):146(2021) [pubmed]

Keyword List

submitter keyword: FLAG-IP, Cryptococcus neoformans

submitter keyword: FLAG-IP, Cryptococcus neoformans

Contact List

Shreyas Sridhar
lab head
Reiko Nakagawa
contact affiliation	RIKEN BDR
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000947/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000947/