PXD021069

PXD021069 is an original dataset announced via ProteomeXchange.

Title	Matrix reverses immortalization-mediated stem cell fate determination
Description	Primary cell culture in vitro suffers from cellular senescence. We hypothesized that expansion on decellularized extracellular matrix (dECM) deposited by simian virus 40 large T antigen (SV40 LT) transduced autologous infrapatellar fat pad stem cells (IPFSCs) could rejuvenate high-passage IPFSCs in both proliferation and chondrogenic differentiation. In the study, we found that SV40 LT transduced IPFSCs exhibited increased proliferation and adipogenic potential but decreased chondrogenic potential. Expansion on dECMs deposited by passage 5 IPFSCs yielded IPFSCs with dramatically increased proliferation and chondrogenic differentiation capacity; however, this enhanced capacity diminished if IPFSCs were grown on dECM deposited by passage 15 IPFSCs. Interestingly, expansion on dECM deposited by SV40 LT transduced IPFSCs yielded IPFSCs with enhanced proliferation and chondrogenic capacity but decreased adipogenic potential, particularly for the dECM group derived from SV40 LT transduced passage 15 cells. Our immunofluorescence staining and proteomics data identify matrix components such as basement membrane proteins top candidates for matrix mediated IPFSC rejuvenation. Both cell proliferation and differentiation were endorsed by transcripts measured by RNASeq during the process. This study provides a promising model for in-depth investigation of matrix protein influence on surrounding stem cell differentiation.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_08:03:40.466.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021069
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ryan Hill
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-23 23:25:25	ID requested
⏵ 1	2022-02-16 08:03:42	announced

Wang Y, Hu G, Hill RC, Dzieciatkowska M, Hansen KC, Zhang XB, Yan Z, Pei M, Matrix reverses immortalization-mediated stem cell fate determination. Biomaterials, 265():120387(2021) [pubmed]

submitter keyword: Infrapatellar fat pad-derived stem cells

Simian virus 40

Proliferation

Chondrogenesis

Decellularized extracellular matrix.

Kirk Hansen
contact affiliation	BMG - University of Colorado - Anschutz Medical Campus
contact email	Kirk.Hansen@cuanschutz.edu
lab head
Ryan Hill
contact affiliation	University of Colorado Anschutz Medical Campus
contact email	Ryan.Hill@ucdenver.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021069
     1. Label: PRIDE project
     2. Name: Matrix reverses immortalization-mediated stem cell fate determination