PXD021063

PXD021063 is an original dataset announced via ProteomeXchange.

Title	Essential role of translation factor eIF5a in cytokine and cell cycle regulation in primary mouse CD8 T lymphocyte
Description	Translation of specific mRNAs is tightly regulated in primary T cells to ensure correct timing and precise delivery of protein syntheses in response to environmental cues. Translation factor eIF5a is known to be important for protein synthesis and is expected to regulate translation of certain difficult mRNA motifs such as consecutive prolines. Functional eIF5a is dynamically regulated upon T cell activation largely through addition of hypusine, a unique post-translational modification, to the mature protein and remarkably the eIF5a family are the only proteins to carry this modification. Here we show that knockout of eIF5a and its hypusination pathway affected both global and gene-specific protein synthesis, especially regulators of cytokine production and cell cycle progression. Furthermore, mRNA targets regulated by eIF5a in activated mouse primary CD8 T cells were systematically identified. AHA-labelled nascent proteins in GC7 (Dhps inhibitor)-treated and eIF5a knockout OT-1 cells (generated using CRSPR-Cas9) were measured using LC-MS. Authors: Thomas CJ Tan, Van Kelly, Xiaoyan Zhou, Tony Ly and Rose Zamoyska
HostingRepository	PRIDE
AnnounceDate	2022-11-11
AnnouncementXML	Submission_2022-11-11_10:05:54.051.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	VanKelly
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-22 16:15:19	ID requested
⏵ 1	2022-11-11 10:05:54	announced

Dataset with its publication pending

submitter keyword: cytokine, lymphocyte, hypusine, proliferation,eIF5a

TonyLy
contact affiliation	Wellcome Centre for Cell Biology, University of Edinburgh
contact email	tony.ly@ed.ac.uk
lab head
VanKelly
contact affiliation	University of Edinburgh
contact email	van.kelly@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021063
     1. Label: PRIDE project
     2. Name: Essential role of translation factor eIF5a in cytokine and cell cycle regulation in primary mouse CD8 T lymphocyte