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PXD021034

PXD021034 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria
DescriptionCells harbor two systems for fatty acid synthesis, one in the cytoplasm (catalyzed by fatty acid synthase, FASN) and one in the mitochondria (mtFAS). In contrast to FASN, mtFAS is poorly characterized, especially in higher eukaryotes, with the major product(s), metabolic roles, and cellular function(s) being essentially unknown. Here we show that hypomorphic mtFAS mutant mouse skeletal myoblast cell lines display a severe loss of electron transport chain (ETC) complexes and exhibit compensatory metabolic activities including reductive carboxylation. This effect on ETC complexes appears to be independent of protein lipoylation, the best characterized function of mtFAS, as mutants lacking lipoylation have an intact ETC. Finally, mtFAS impairment blocks the differentiation of skeletal myoblasts in vitro. Together, these data suggest that ETC activity in mammals is profoundly controlled by mtFAS function, thereby connecting anabolic fatty acid synthesis with the oxidation of carbon fuels.
HostingRepositoryPRIDE
AnnounceDate2020-09-02
AnnouncementXMLSubmission_2020-09-02_07:07:23.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJoao Paulo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-20 22:54:46ID requested
12020-09-02 07:07:24announced
Publication List
Nowinski SM, Solmonson A, Rusin SF, Maschek JA, Bensard CL, Fogarty S, Jeong MY, Lettlova S, Berg JA, Morgan JT, Ouyang Y, Naylor BC, Paulo JA, Funai K, Cox JE, Gygi SP, Winge DR, DeBerardinis RJ, Rutter J, Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria. Elife, 9():(2020) [pubmed]
Keyword List
submitter keyword: Mitochondria, fatty acid synthesis, electron transport chain, reductive carboxylation, lipoylation TCA cycle, metabolism, respiration, OXPHOS, muscle
Contact List
Steven P. Gygi
contact affiliationDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA
contact emailsgygi@hms.harvard.edu
lab head
Joao Paulo
contact affiliationHarvard Medical School
contact emailjoao_paulo@post.harvard.edu
dataset submitter
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PRIDE project URI
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