PXD021024

PXD021024 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistant of Peppers (Capsicum annuum L.)
Description	Lysine acetylation in proteins is a dynamic and reversible Post-translational modification and plays an important role in diverse cellular processes, but limited knowledge is available for acetylation modifications in pepper (Capsicum annuum L.) resistance of cold stress. In this study, the proteome and acetylome of two peppers with different cold resistance under different cold stress and recovery treatment were investigated. In total, 6213 proteins groups and 4574 lysine acetylation sites of 2261 protein groups were identified. Cold stress and recovery treatment results in 3008 differentially expressed proteins (DEPs) and 768 differentially expressed acetylated proteins (DEAPs). Further analysis found a total 1988 proteins were both identifed in the proteome and acetylome data and elucidated the functional differences between the up-regulated and down-regulated proteins in these co-identified proteins through GO enrichment. Twenty acetylation motifs were defined on 3934 unique lysine acetylation sites and motifs *KS*, *KY*, and *KH* occupied the highest proportion of all the identified peptides. Subcellular distribution predictions showed that acetylated proteins in pepper leaves distributed predominantly in chloroplast, cytoplasm and nuclear. KEGG analysis showed 397 identified acetylated proteins were involved in 93 different metabolic pathways. Then the dynamic changes of acetylated proteins in photosynthesis and carbon fixation in photosynthetic organisms pathways under cold stress were further analyzed and many key acetylated proteins regulating cold resistance of pepper were found in these two metabolic pathways. This study is the first to identify the acetylome in pepper, expands greatly the catalog of lysine acetylation substrates and sites in Solanaceae crops and provide a new insight for the post-translational modification study.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_04:11:17.468.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhoubin Liu
SpeciesList	scientific name: Capsicum annuum; NCBI TaxID: 4072;
ModificationList	acetylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-20 08:24:23	ID requested
⏵ 1	2022-02-16 04:11:18	announced

Liu Z, Song J, Miao W, Yang B, Zhang Z, Chen W, Tan F, Suo H, Dai X, Zou X, Ou L, L.). Front Plant Sci, 12():730489(2021) [pubmed]

submitter keyword: Pepper

proteome

lysine acetylation

cold stress

recovery

photosynthesis

carbon fixation in photosynthetic organisms

Zhoubin Liu
contact affiliation	College of Horticulture, Hunan Agricultural University
contact email	hnliuzhoubin@163.com
lab head
zhoubin Liu
contact affiliation	College of Horticulture, Hunan Agricultural University
contact email	hnliuzhoubin@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021024
     1. Label: PRIDE project
     2. Name: Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistant of Peppers (Capsicum annuum L.)