PXD021023

PXD021023 is an original dataset announced via ProteomeXchange.

Title	Investigating byssogenesis with proteomic analysis of byssus, foot and mantle in Mytilus mussels by LC-MS/MS
Description	Mussels synthesize an interesting class of biological materials with unique properties to adhere onto virtually any solid surface. Inter- and cross-species analyses have revealed that mussel byssus fabrication is influenced by environmental factors. In this study, proteins expressed specifically byssus and in the byssal-producing organ (foot) were examined for two mussel species from the Mytilidae family using liquid chromatography coupled with high-resolution tandem mass spectrometry (LC-MS/MS). The main goal was to describe which proteins are exclusively expressed in the mussel's foot gland, which is responsible for byssus secretion. Proteins uniquely found in the foot samples have been found by comparing to a control tissue (mantle). The overlap with proteins found in byssus samples were also investigated. A restricted list of these proteins have been highlighted as byssus-related, believed to contribute to the unique properties of byssus, such as securing adhesion and toughness, and plasticity based on environmental factors (including promoter regulation). This work may provide further directions on thread biofabrication and additional information on its regulation, as well as contribute to a more complete description of the mussel proteome.
HostingRepository	PRIDE
AnnounceDate	2020-09-17
AnnouncementXML	Submission_2020-09-17_03:39:10.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021023
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lekha Sleno
SpeciesList	scientific name: Mytilus californianus; NCBI TaxID: 6549; scientific name: Mytilus edulis; NCBI TaxID: 6550;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-20 04:15:26	ID requested
⏵ 1	2020-09-17 03:39:11	announced

Sansoucy M, Tremblay R, Carrington E, Marcotte I, Sleno L, Investigating Byssogenesis with Proteomic Analysis of Byssus, Foot, and Mantle in Mytilus Mussels by LC-MS/MS. Proteomics, 21(2):e2000014(2021) [pubmed]

submitter keyword: Mussel, byssus, foot, LC-MS/MS, bottom-up proteomics

Lekha Sleno
contact affiliation	Bioanalytical Mass Spectrometry Research Group, Chemistry Department, Université du Québec à Montréal (UQAM) Montréal (Québec) Canada (lab head)
contact email	sleno.lekha@uqam.ca
lab head
Lekha Sleno
contact affiliation	Université du Québec à Montréal (UQÀM), Chemistry Department / Pharmaqam, Montréal, QC, Canada
contact email	sleno.lekha@uqam.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021023
     1. Label: PRIDE project
     2. Name: Investigating byssogenesis with proteomic analysis of byssus, foot and mantle in Mytilus mussels by LC-MS/MS