PXD021016

PXD021016 is an original dataset announced via ProteomeXchange.

Title	Murine alveolar macrophage cell line MH-S was transduced with lentivirus expressing GFP alone or GFP plus murine MFSD7C tagged with Myc and FLAG epitopes.
Description	We used immunoprecipitation (IP) and mass spectrometry (MS) to identify proteins that interact with MFSD7C. Because an anti-MFSD7C monoclonal antibody is not available, we used MFSD7C tagged with both Myc and FLAG epitopes in a MFSD7C-expressing murine alveolar macrophage cell line MH-S (Supplementary Fig. 2a-c and see Methods for details). A total of 58 proteins were identified excluding MFSD7C and common contaminants of IP experiments such as keratin, actin, and ribosomal proteins (Table 1). Twenty-six of the 58 proteins are annotated as mitochondrial proteins and 4 (three Fc receptors and one integrin) are known to reside in the cytoplasmic membrane. The 58 proteins were classified into 10 different functional categories based on gene ontology.
HostingRepository	PRIDE
AnnounceDate	2020-08-20
AnnouncementXML	Submission_2020-09-29_23:29:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yingzhong Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-19 23:41:15	ID requested
1	2020-08-20 03:31:34	announced
⏵ 2	2020-09-29 23:29:57	announced	2020-09-30: Updated publication reference for PubMed record(s): 32973183.

Li Y, Ivica NA, Dong T, Papageorgiou DP, He Y, Brown DR, Kleyman M, Hu G, Chen WW, Sullivan LB, Del Rosario A, Hammond PT, Vander Heiden MG, Chen J, MFSD7C switches mitochondrial ATP synthesis to thermogenesis in response to heme. Nat Commun, 11(1):4837(2020) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Mitochondria (HPP)

submitter keyword: Murine, Alveolar macropahge, MFSD7C, LC-MSMS

Jianzhu Chen
contact affiliation	Koch Institute for Integrative Cancer Research at Massachusetts Institute of Technology
contact email	jchen@mit.edu
lab head
Yingzhong Li
contact affiliation	The Koch Institute for Integrative Cancer Research at MIT
contact email	lyzmit@mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD021016

PRIDE project URI

• PRIDE
  1. PXD021016
     1. Label: PRIDE project
     2. Name: Murine alveolar macrophage cell line MH-S was transduced with lentivirus expressing GFP alone or GFP plus murine MFSD7C tagged with Myc and FLAG epitopes.