PXD021007

PXD021007 is an original dataset announced via ProteomeXchange.

Title	Phomopsis amygdala fusicoccadiene synthase
Description	Fusicoccadiene synthase from Phomopsis amygdali (PaFS) catalyzes the first committed step in the biosynthesis of Fusicoccin A, a diterpene glycoside and a a potential therapeutic agent. PaFS contains a C-terminal prenyltransferase domain that generates geranylgeranyl diphosphate (GGPP) and an N-terminal cyclase domain that utilizes GGPP to generate fusicoccadiene, which comprises the complex 5-8-5 tricyclic hydrocarbon skeleton of Fusicoccin A. The C-termimal domain octamerizes to form a central core, with the eight cyclase domains radiating outward via flexible linker segments with variable extended conformations. Crosslinking-mass spectrometry (XL-MS) experiments affirm and reveal inter-domain interactions, showing that compact conformations can be achieved. These studies, complemented by cryo-EM and functional studies, provide a framework for understanding substrate channeling between consecutive catalytic domains.
HostingRepository	PRIDE
AnnounceDate	2021-07-07
AnnouncementXML	Submission_2021-07-06_22:34:33.166.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	hee jong kim
SpeciesList	scientific name: Phomopsis sp. amygdali NY7157a; NCBI TaxID: 872227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-19 08:36:00	ID requested
⏵ 1	2021-07-06 22:34:33	announced

Faylo JL, van Eeuwen T, Kim HJ, Gorbea Col, ó, n JJ, Garcia BA, Murakami K, Christianson DW, Structural insight on assembly-line catalysis in terpene biosynthesis. Nat Commun, 12(1):3487(2021) [pubmed]

submitter keyword: GGPP synthase, terpene cyclase

benjamin aaron garcia
contact affiliation	University of Pennsylvania
contact email	bgarci@pennmedicine.upenn.edu
lab head
hee jong kim
contact affiliation	university of pennsylvania, Garcia Lab, Murakami Lab
contact email	heejong@heejong.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD021007

PRIDE project URI

• PRIDE
  1. PXD021007
     1. Label: PRIDE project
     2. Name: Phomopsis amygdala fusicoccadiene synthase