PXD020984

PXD020984 is an original dataset announced via ProteomeXchange.

Title	SILAC analysis of exosome secretion in response to centrosome amplification
Description	We performed quantitative proteomics analysis by Stable Isotope Labelling by Amino Acids in Cell culture (SILAC) to understand how centrosome amplification changes the composition of human small extracellular vesicles. We carried out SILAC labelling with medium and heavy isotopes, as it enables the exclusion of contaminant serum proteins, which would be unlabeled (equivalent of light labeling), as well as allowing for simultaneous processing of purification steps to decrease sample-to-sample variability. We isolated small extracellular vesicles by ultracentifugation followed by size exclusion chromatography (SEC). All experiments were performed in duplicate with switched SILAC labelling.
HostingRepository	PRIDE
AnnounceDate	2021-01-19
AnnouncementXML	Submission_2021-01-19_02:26:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Faraz Mardakheh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-18 22:48:02	ID requested
⏵ 1	2021-01-19 02:26:43	announced

Dataset with its publication pending

submitter keyword: SILAC, centrosome amplification, exosomes, small extracellular vesicles.

Faraz Mardakheh
contact affiliation	Centre for Cancer Cell and Molecular Biology Barts Cancer Institute, Queen Mary University of London Charterhouse Square, London EC1M 6BQ, The United Kingdom.
contact email	f.mardakheh@qmul.ac.uk
lab head
Faraz Mardakheh
contact affiliation	Barts Cancer Institute
contact email	f.mardakheh@qmul.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD020984

PRIDE project URI

• PRIDE
  1. PXD020984
     1. Label: PRIDE project
     2. Name: SILAC analysis of exosome secretion in response to centrosome amplification