PXD020983

PXD020983 is an original dataset announced via ProteomeXchange.

Title	Dataset of the proteome profiles of sea bass (Dicentrarchus labrax) skin-scale exposed to estradiol and fluoxetine
Description	Contamination of aquatic ecosystems with anthropogenic pollutants, including pharmaceutical drugs, is a major concern worldwide. Fish are particularly at risk of exposure to pollutants but their impacts on mineralized fish tissues, particularly the scales that form a barrier between the fish and the environment, are poorly understood. The proteome data here presented support the findings reported in the associated research article “Disruption of the sea bass (Dicentrarchus labrax) skin-scale by estradiol and fluoxetine, an emerging pollutant”. Juvenile sea basses were exposed by intraperitoneal injections to: a) the antidepressant fluoxetine (FLX), a widely prescribed psychotropic drug and an emerging pollutant; b) the natural estrogen 17β-estradiol (E2) and c) coconut oil alone (control). The scale proteome profiles of fish exposed to these compounds for 5 days were analysed by the quantitative label-free proteomics technology SWATH-MS (Sequential Windowed data-independent Acquisition of the Total High-resolution-Mass Spectra). LC-MS data from pooled protein extracts from the scales of all experimental groups were acquired using information-dependent acquisition (IDA), which allowed the identification of 1,254 proteins through searches against the sea bass genome database. 715 proteins were confidently quantified by SWATH acquisition, from which 213 proteins had modified levels (p<0.05) between E2- or FLX-exposed fish and control. The main biological processes and KEGG pathways affected by E2 or FLX treatments were identified using Cytoscape/ClueGO enrichment analyses.
HostingRepository	PRIDE
AnnounceDate	2022-01-11
AnnouncementXML	Submission_2022-03-14_03:16:47.945.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cátia Santa
SpeciesList	scientific name: Dicentrarchus labrax; NCBI TaxID: 13489;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-18 22:34:44	ID requested
1	2022-01-11 05:14:16	announced
⏵ 2	2022-03-14 03:16:50	announced	2022-03-14: Updated publication reference for PubMed record(s): 35252491.

Anjos L, Pinto PIS, Santos S, Est, ê, v, ã, o MD, Santa C, Manadas B, Can, á, rio AVM, Power DM, ) skin-scales exposed to fluoxetine and estradiol. Data Brief, 41():107971(2022) [pubmed]

submitter keyword: SWATH-MS quantitative proteomics

fish scales

emerging pollutants

antidepressant

estrogen;

Bruno Manadas
contact affiliation	Center for Neuroscience and Cell Biology of the University of Coimbra
contact email	bmanadas@gmail.com
lab head
Cátia Santa
contact affiliation	Center for Neuroscience and Cell Biology
contact email	catiajmsanta@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD020983

PRIDE project URI

• PRIDE
  1. PXD020983
     1. Label: PRIDE project
     2. Name: Dataset of the proteome profiles of sea bass (Dicentrarchus labrax) skin-scale exposed to estradiol and fluoxetine