PXD020982

PXD020982 is an original dataset announced via ProteomeXchange.

Title	MS/MS analysis of Ub-conjugated UbcH7 ubiquitination sites
Description	By conducting the ubiquitin-conjugation assay for UbcH7, we found that PknG was able to promote the loading of Ub onto UbcH7, which is indicated by a molecular weight shift in SDS–PAGE. The classic Ub conjugation of E2s requires the E1 to form a thiol-ester bond between its active Cys and the C-terminal Gly of Ub. Notably, PknG could directly catalyze Ub conjugation onto UbcH7 in a kinase activity-independent and Ubl domain-dependent manner.
HostingRepository	PRIDE
AnnounceDate	2021-04-16
AnnouncementXML	Submission_2021-04-16_03:35:01.253.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lu z
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-18 22:32:11	ID requested
⏵ 1	2021-04-16 03:35:01	announced

Dataset with its publication pending

submitter keyword: MS, unconventional isopeptidase activitys

Cuihua Liu
contact affiliation	CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;
contact email	wangj6@im.ac.cn
lab head
lu z
contact affiliation	Institute of Microbiology
contact email	lu1457883143@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD020982

PRIDE project URI

• PRIDE
  1. PXD020982
     1. Label: PRIDE project
     2. Name: MS/MS analysis of Ub-conjugated UbcH7 ubiquitination sites