PXD020970

PXD020970 is an original dataset announced via ProteomeXchange.

Title	Cellular proteins interacting with curved HIV-1 capsid lattices
Description	Early events of HIV-1 lifecycle, such as post-entry virus trafficking, uncoating and nuclear import, remain poorly understood due to limited information about virus-host interactions. We used a multidisciplinary approach. First, a mass spectrometry (MS)-based proteomics methodology enabled us to identify cellular proteins that specifically bind to 3-dimensional curved hexameric capsid lattices closely mimicking the assembled HIV-1 core. We then employed virology, live cell imaging, CRISPR gene knockout, x-ray crystallography, and biochemistry to demonstrate that Sec24C (a component of the COPII complex) is the first example of a cytoplasmic protein that utilizes an FG containing motif to directly and specifically bind the curved hexameric HIV-1 capsid lattices.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_12:58:58.227.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020970
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nikoloz Shkriabai
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap XL; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-18 04:12:22	ID requested
⏵ 1	2021-03-04 12:58:58	announced

Dataset with its publication pending

submitter keyword: HIV-1, capsid, human proteins, proteomics

Mamuka Kvaratskhelia
contact affiliation	Mamuka Kvaratskhelia, University of Colorado School of Medicine, Division of Infectious Diseases, 12700 E. 19th Avenue, Research Complex 2, Aurora, CO 80045, USA
contact email	mamuka.kvaratskhelia@cuanschutz.edu
lab head
Nikoloz Shkriabai
contact affiliation	University of Colorado
contact email	nikoloz.shkriabai@cuanschutz.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD020970

PRIDE project URI

• PRIDE
  1. PXD020970
     1. Label: PRIDE project
     2. Name: Cellular proteins interacting with curved HIV-1 capsid lattices