PXD020969

PXD020969 is an original dataset announced via ProteomeXchange.

Title	Nuclear receptor chemical reporter enables domain-specific analysis of ligands in mammalian cells
Description	The characterization of specific metabolite–protein interactions is important in chemical biology and drug discovery. For example, nuclear receptors (NRs) are a family of ligand-activated transcription factors that regulate diverse physiological processes in animals and are key targets for therapeutic development. However, the identification and characterization of physiological ligands for many NRs remains challenging due to limitations in domain-specific analysis of ligand binding in cells. To address these limitations, we developed a domain-specific covalent chemical reporter for peroxisome proliferator–activated receptors (PPARs) and demonstrated its utility to screen and characterize the potency of candidate NR ligands in live cells. These studies demonstrate that targeted and domain-specific chemical reporters provide excellent tools to evaluate endogenous and exogenous (diet, microbiota, therapeutics) ligands of PPARs in mammalian cells as well as additional protein targets for further investigation.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_03:17:15.857.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taku Tsukidate
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-18 04:06:30	ID requested
⏵ 1	2021-09-09 03:17:16	announced

Tsukidate T, Li Q, Hang HC, Nuclear Receptor Chemical Reporter Enables Domain-Specific Analysis of Ligands in Mammalian Cells. ACS Chem Biol, 15(9):2324-2330(2020) [pubmed]

submitter keyword: LC-MSMS

Howard C Hang
contact affiliation	Departments of Immunology and Microbiology, Chemistry, Scripps Research, La Jolla, California 92037, United States
contact email	hhang@scripps.edu
lab head
Taku Tsukidate
contact affiliation	The Rockefeller University
contact email	ttsukidate@rockefeller.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020969

PRIDE project URI

• PRIDE
  1. PXD020969
     1. Label: PRIDE project
     2. Name: Nuclear receptor chemical reporter enables domain-specific analysis of ligands in mammalian cells