⮝ Full datasets listing

PXD020968

PXD020968 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic adaptation of Streptococcus pneumoniae to the antimicrobial peptide human Beta Defensin 3 (hBD3) in comparison to other cell surface stresses
DescriptionThe antimicrobial peptide human Beta Defensin 3 (hBD3) is an essential part of the innate immune system and involved in protection against respiratory pathogens by specifically permeabilizing bacterial membranes. The Gram-positive bacterium S. pneumoniae causes serious diseases including pneumonia, meningitis, and septicemia despite being frequently exposed to human defense molecules including hBD3 during colonization and infection. Thus, the question arises how pneumococci adapt to stress caused by antimicrobial peptides. We addressed this subject by analyzing the proteome of S. pneumoniae after treatment with hBD3 and compared our data with the proteomic changes induced by LL-37, another crucial antimicrobial peptide present in the human respiratory tract. As antimicrobial peptides usually cause membrane perturbations, the response to the membrane active cationic detergent cetyl trimethyl ammonium bromide (CTAB) was examined to assess the specificity of the pneumococcal response to antimicrobial peptides. In brief, hBD3 and LL-37 induce a similar response in pneumococci especially in changes of proteins with annotated transporter and virulence function. However, LL-37 causes changes in abundance of cell surface modification proteins that cannot be observed after treatment with hBD3. Interestingly, CTAB induces unique proteomic changes in S. pneumoniae. Though, the detergent seems to activate a two-component system that is also activated in response to antimicrobial peptide stress (TCS 05). Overall, our publicly available data represent a novel resource on pneumococcal adaptation to specific cell surface stresses on a functional level. This knowledge can potentially be used to develop strategies to circumvent pneumococcal resistance to antimicrobial peptides.
HostingRepositoryPRIDE
AnnounceDate2020-11-02
AnnouncementXMLSubmission_2020-11-04_23:17:17.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPierre-Alexander Mücke
SpeciesList scientific name: Streptococcus pneumoniae D39; NCBI TaxID: 373153;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-18 04:01:52ID requested
12020-11-01 22:44:33announced
22020-11-02 00:01:18announced2020-11-02: Updated project metadata.
32020-11-04 23:17:18announced2020-11-02: Updated project metadata.
2020-11-05: Updated publication reference for PubMed record(s): 33143252.
Publication List
M, ü, cke PA, Ostrzinski A, Hammerschmidt S, Maa, ß S, Becher D, to the Antimicrobial Peptide Human Beta Defensin 3 (hBD3) in Comparison to Other Cell Surface Stresses. Microorganisms, 8(11):(2020) [pubmed]
Keyword List
submitter keyword: Streptococcus pneumoniae, antimicrobial peptides, hBD3, LL-37, adaptation, proteomics
Contact List
Dörte Becher
contact affiliationDepartment of Microbial Proteomics, Institute of Microbiology, Center for Functional Genomics of Microbes, University of Greifswald, Felix-Hausdorff-Straße 8, 17489 Greifswald, Germany
contact emaildbecher@uni-greifswald.de
lab head
Pierre-Alexander Mücke
contact affiliationDepartment of Microbial Proteomics, Institute of Microbiology, Center for Functional Genomics of Microbes, University of Greifswald, Felix-Hausdorff-Straße 8, 17489 Greifswald, Germany
contact emailpierre.muecke@uni-greifswald.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD020968
PRIDE project URI
Repository Record List
[ + ]