PXD020967

PXD020967 is an original dataset announced via ProteomeXchange.

Title	SARS-COV-2 Uses CD4 to infect T Helper Lymphocytes
Description	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 or CoV-2) is the agent of a major global outbreak of respiratory tract disease known as COVID-19. CoV-2 infects the lungs and may cause several immune-related complications such as lymphocytopenia and cytokine storm which are associated with the severity of the disease and predict mortality. The mechanism by which CoV-2 infection may result in immune system dysfunction is not fully understood. Here we showed that CoV-2 infects human CD4+ T helper cells, but not CD8+ T cells. CoV-2 is found in the blood and bronchoalveolar lavage T helper cells of severe COVID-19 patients. We demonstrated that CoV-2 spike glycoprotein (S) (sCoV-2) directly binds to the CD4 co-receptor, which in turn mediates the entry of CoV-2 in T helper cells in a mechanism that also requires ACE2 and TMPRSS2. Once inside T helper cells, CoV-2 assembles viral factories, impairs cell function and may cause cell death. CoV-2 infected T helper cells express higher amounts of IL-10, which has been associated with viral persistence and disease severity. Thus, CD4-mediated CoV-2 infection of T helper cells may explain the poor adaptive immune response of many COVID-19 patients.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:48:27.049.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020967
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Victor Corasolla Carregari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-17 22:48:05	ID requested
1	2023-07-28 08:26:58	announced
⏵ 2	2023-11-14 06:48:27	announced	2023-11-14: Updated project metadata.

10.6019/PXD020967;

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: LC-MSMS, T-Cell, Sars-Cov-2 infection,Proteomics

Daniel Martins-de-Souza
contact affiliation	Associate Professor at University of Campinas, Sao Paulo-Brazil (UNICAMP-SP.
contact email	danms90@gmail.com
lab head
Victor Corasolla Carregari
contact affiliation	Post doctor
contact email	corasolla@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD020967

PRIDE project URI

• PRIDE
  1. PXD020967
     1. Label: PRIDE project
     2. Name: SARS-COV-2 Uses CD4 to infect T Helper Lymphocytes