PXD020943

PXD020943 is an original dataset announced via ProteomeXchange.

Title	NIK expression stimulated G6PD phosphorylation
Description	Our findings establish NIK as a pivotal regulator of T cell metabolism in anti-tumor immunity and highlight a posttranslational mechanism of metabolic regulation involving the G6PD-NADPH redox system. CoIP assays revealed a strong physical interaction between NIK and G6PD in both T cells and transiently transfected 293 cells, suggesting G6PD to be a direct target of NIK. Using a phosphoprotein gel analysis approach, we demonstrated that NIK expression stimulated G6PD phosphorylation. To further study the mechanism, we performed mass spectrometry identify phosphorylation sites of G6PD stimulated by NIK
HostingRepository	PRIDE
AnnounceDate	2020-10-05
AnnouncementXML	Submission_2020-10-05_06:53:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020943
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Meidi Gu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-17 02:50:33	ID requested
⏵ 1	2020-10-05 06:53:38	announced

Dataset with its publication pending

submitter keyword: G6PD,NIK,phosphoralytion

David H. Hawke, Ph.D.
contact affiliation	U.T. MD Anderson Cancer Center Department of Systems Biology,Proteomics Facility, Unit 950 Room 2SCR3.3123 The UT-M.D. Anderson Proteomics Facility would like to thank the M.D. Anderson Cancer Center, and the NIH High-End Instrumentation program grant 1S10OD012304-01 for generous support.
contact email	dhawke@mdanderson.org
lab head
Meidi Gu
contact affiliation	MD Anderson Cancer Center
contact email	mgu2@mdanderson.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020943
     1. Label: PRIDE project
     2. Name: NIK expression stimulated G6PD phosphorylation