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PXD020942

PXD020942 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleContext-enriched interactome powered by proteomics helps the identification of novel regulators of macrophage activation
DescriptionAbstract: The role of pro-inflammatory macrophage activation in cardiovascular disease (CVD) is a complex one amenable to network approaches. While an indispensible tool for elucidating the molecular underpinnings of complex diseases including CVD, the interactome is limited in its utility as it is not specific to any cell type, experimental condition or disease state. We introduced context-specificity to the interactome by combining it with co-abundance networks derived from unbiased proteomics measurements from activated macrophage-like cells. Each macrophage phenotype contributed to certain regions of the interactome. Using a network proximity-based prioritization method on the combined network, we predicted potential regulators of macrophage activation. Prediction performance significantly increased with the addition of co-abundance edges, and the prioritized candidates captured inflammation, immunity and CVD signatures. Integrating the novel network topology with transcriptomics and proteomics revealed top candidate drivers of inflammation. In vitro loss-of-function experiments demonstrated the regulatory role of these proteins in pro-inflammatory signaling.
HostingRepositoryPRIDE
AnnounceDate2020-08-18
AnnouncementXMLSubmission_2020-08-17_22:33:14.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSasha Singh
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-17 02:45:23ID requested
12020-08-17 22:33:16announced
Publication List
Halu A, Wang JG, Iwata H, Mojcher A, Abib AL, Singh SA, Aikawa M, Sharma A, Context-enriched interactome powered by proteomics helps the identification of novel regulators of macrophage activation. Elife, 7():(2018) [pubmed]
Iwata H, Goettsch C, Sharma A, Ricchiuto P, Goh WW, Halu A, Yamada I, Yoshida H, Hara T, Wei M, Inoue N, Fukuda D, Mojcher A, Mattson PC, Barab, รก, si AL, Boothby M, Aikawa E, Singh SA, Aikawa M, PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation. Nat Commun, 7():12849(2016) [pubmed]
Keyword List
submitter keyword: THP-1 cells, TMT, macrophages,
Contact List
Masanori Aikawa
contact affiliationBrigham and Women's Hospital
contact emailmaikawa@bwh.harvard.edu
lab head
Sasha Singh
contact affiliationBrigham and Women's Hospital, Harvard Medical School
contact emailsasingh@bwh.harvard.edu
dataset submitter
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Dataset FTP location
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