PXD020940

PXD020940 is an original dataset announced via ProteomeXchange.

Title	Oryza sativa cv. Nipponbare and Oryza barthii as unexpected tolerance and susceptibility sources against Schizotetranychus oryzae (Acari: Tetranychidae) mite infestation
Description	Cultivated rice (Oryza sativa L.) is frequently exposed to multiple stresses, including Schizotetranychus oryzae mite infestation. Rice domestication has narrowed the genetic diversity of the species, leading to a wide susceptibility. This work aimed to observe the response of two wild rice species (Oryza barthii and O. glaberrima) and two O. sativa genotypes (cv. Nipponbare and f. spontanea) to S. oryzae infestation. Surprisingly, leaf damage, histochemistry, chlorophyll concentration and fluorescence showed that the wild species present higher level of leaf damage, increased accumulation of H2O2 and lower photosynthetic capacity when compared to O. sativa genotypes under infested conditions. Infestation decreased tiller number, except in Nipponbare. Infestation also caused the death of wild plants during the reproductive stage. While infestation did not affect the weight of 1,000 grains in both O. sativa genotypes, the number of panicles per plant was affected only in f. spontanea, and the percentage of full seeds per panicle and seed length were increased only in Nipponbare. Using proteomic analysis, we identified 195 differentially abundant proteins when comparing susceptible (O. barthii) and tolerant (Nipponbare) genotypes under control and infested conditions. O. barthii has a less abundant antioxidant arsenal and is unable to modulate proteins involved with general metabolism and energy production under infested condition. Nipponbare presents high abundance of detoxification-related proteins, general metabolic processes and energy production, suggesting that, under infested condition, the primary metabolism is maintained more active compared to O. barthii. Also, under infested conditions, Nipponbare presents higher levels of proline and a greater abundance of defense-related proteins, such as osmotin, ricin B-like lectin, and protease inhibitors. These differentially abundant proteins can be used as biotechnological tools in breeding programs aiming increased tolerance to mite infestation.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_02:46:20.612.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Raul Sperotto
SpeciesList	scientific name: Oryza barthii; NCBI TaxID: 65489; scientific name: Oryza sativa; NCBI TaxID: 4530;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-17 00:21:38	ID requested
⏵ 1	2021-09-09 02:46:21	announced

Buffon G, Blasi É ADR, Lamb TI, Adamski JM, Schwambach J, Ricachenevsky FK, Bertolazi A, Silveira V, Lopes MCB, Sperotto RA, (Acari: Tetranychidae) Mite Infestation. Front Plant Sci, 12():613568(2021) [pubmed]

submitter keyword: Osmotin, proline, protease inhibitors, resistance, wild species

Raul Antonio Sperotto
contact affiliation	Biological Sciences and Health Center, University of Taquari Valley - Univates, Lajeado, RS, Brazil
contact email	rasperotto@univates.br
lab head
Raul Sperotto
contact affiliation	University of Taquari Valley - Univates
contact email	rasperotto@univates.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020940
     1. Label: PRIDE project
     2. Name: Oryza sativa cv. Nipponbare and Oryza barthii as unexpected tolerance and susceptibility sources against Schizotetranychus oryzae (Acari: Tetranychidae) mite infestation