PXD020932

PXD020932 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein binding to PDCD4 promoter X-box in Nutlin-3a treated U2OS cells
Description	Human PDCD4 wild-type (wt) promoter fragments were amplified from U2OS genomic DNA and X-box deletion mutants (mut) were generated using the QuikChange site-directed mutagenesis kit (Agilent Technologies). DNA probes for affinity purification with the same sequences were obtained by PCR using a biotinylated primer for labeling the 3' end (Thermo Fisher Scientific). DNA affinity purification with nuclear extracts from Nutlin-3a treated U2OS cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:53:22.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Ori
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-16 22:55:10	ID requested
1	2024-08-08 10:10:17	announced
⏵ 2	2024-10-22 06:53:22	announced	2024-10-22: Updated project metadata.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: proteomics, RFX7,human, p53

submitter keyword: proteomics, RFX7,human, p53

Contact List

Martin Fisher
contact affiliation	Leibniz Institute on Aging – Fritz Lipmann Institute (FLI)Beutenbergstr. 11 07745 Jena Germany Tel.: +49 3641 656876
contact email	Martin.Fischer@leibniz-fli.de
lab head
Alessandro Ori
contact affiliation	Leibniz Institute on Aging
contact email	aleori@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD020932
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD020932

PRIDE project URI

Repository Record List

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