PXD020932 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein binding to PDCD4 promoter X-box in Nutlin-3a treated U2OS cells |
Description | Human PDCD4 wild-type (wt) promoter fragments were amplified from U2OS genomic DNA and X-box deletion mutants (mut) were generated using the QuikChange site-directed mutagenesis kit (Agilent Technologies). DNA probes for affinity purification with the same sequences were obtained by PCR using a biotinylated primer for labeling the 3' end (Thermo Fisher Scientific). DNA affinity purification with nuclear extracts from Nutlin-3a treated U2OS cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:53:22.107.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alessandro Ori |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-16 22:55:10 | ID requested | |
1 | 2024-08-08 10:10:17 | announced | |
⏵ 2 | 2024-10-22 06:53:22 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, RFX7,human, p53 |
Contact List
Martin Fisher |
contact affiliation | Leibniz Institute on Aging – Fritz Lipmann Institute (FLI)Beutenbergstr. 11 07745 Jena Germany Tel.: +49 3641 656876 |
contact email | Martin.Fischer@leibniz-fli.de |
lab head | |
Alessandro Ori |
contact affiliation | Leibniz Institute on Aging |
contact email | aleori@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020932
- Label: PRIDE project
- Name: Protein binding to PDCD4 promoter X-box in Nutlin-3a treated U2OS cells