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PXD020932

PXD020932 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein binding to PDCD4 promoter X-box in Nutlin-3a treated U2OS cells
DescriptionHuman PDCD4 wild-type (wt) promoter fragments were amplified from U2OS genomic DNA and X-box deletion mutants (mut) were generated using the QuikChange site-directed mutagenesis kit (Agilent Technologies). DNA probes for affinity purification with the same sequences were obtained by PCR using a biotinylated primer for labeling the 3' end (Thermo Fisher Scientific). DNA affinity purification with nuclear extracts from Nutlin-3a treated U2OS cells.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:53:22.107.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlessandro Ori
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-16 22:55:10ID requested
12024-08-08 10:10:17announced
22024-10-22 06:53:22announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, RFX7,human, p53
Contact List
Martin Fisher
contact affiliationLeibniz Institute on Aging – Fritz Lipmann Institute (FLI)Beutenbergstr. 11 07745 Jena Germany Tel.: +49 3641 656876
contact emailMartin.Fischer@leibniz-fli.de
lab head
Alessandro Ori
contact affiliationLeibniz Institute on Aging
contact emailaleori@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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