PXD020922

PXD020922 is an original dataset announced via ProteomeXchange.

Title	PRM-based quantitative proteomic analysis of mouse tissues, testicular cells and cell lines
Description	PRM analysis of RPL39L, RPL10L and RPL3L in 9 mouse tissues, including testis, fat, brain, liver, kidney, lung, skeletal muscle, heart and spleen; PRM analysis of RPL39 and RPL39L, RPL10 and RPL10L, and RPL22 and RPL22L1 in testicular cells; PRM analysis of RPL39L and RPL39 in RibosomeRPL39L-/- and wild type mouse spermatocytes and spermatids; PRM analysis of 17 down-regulated proteins and 1 unchanged protein (DYNC1LI2) in RibosomeRPL39L-/- testes; PRM analysis of RPL39 and RPL39L in GC-1 ribosomes, and RibosomeRPL39L-/- and wild type N2a ribosomes, Consistency between the observed and expected signal ratios of the light and heavy peptides by PRM in serial dilution experiments. Bovine serum albumin (BSA) as a negative control.
HostingRepository	PRIDE
AnnounceDate	2022-09-30
AnnouncementXML	Submission_2022-09-30_01:06:39.879.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yao liping
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-15 16:12:25	ID requested
⏵ 1	2022-09-30 01:06:40	announced

Dataset with its publication pending

submitter keyword: PRM, quantification，testicular tissues

Xuejiang Guo
contact affiliation	State Key Laboratory of Reproductive Medicine. Department of Histology and Embryology, Nanjing Medical University, Nanjing, China. (lab head)
contact email	guo_xuejiang@njmu.edu.cn
lab head
Yao liping
contact affiliation	18351998967
contact email	2356707339@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD020922

PRIDE project URI

• PRIDE
  1. PXD020922
     1. Label: PRIDE project
     2. Name: PRM-based quantitative proteomic analysis of mouse tissues, testicular cells and cell lines