PXD020908

PXD020908 is an original dataset announced via ProteomeXchange.

Title	Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation
Description	Human periodontal ligament stem cells (hPDLSCs) are ideal seed cells for periodontal regeneration. To better understand the dynamic protein profiles during hPDLSCs osteogenic differentiation, we conducted a quantitative proteomics to reveal the temporal protein expression pattern during this process. Clustering and functional annotation by Gene Ontology terms identified distinctions among proteins with different dynamic tendencies. Pathway enrichment analysis with the Kyoto Encyclopedia of Genes and Genomes database found that oxidative phosphorylation (OXPHOS) was significantly involved, which further predicted continuous activation by Ingenuity Pathway Analysis software. Redox-sensitive signalling pathways with dynamic activation status showed associations with OXPHOS to various degrees, especially the sirtuin signalling pathway. SOD2, an important component of the sirtuin signalling pathway, is a hub protein of the interaction network and displays a persistent increase during osteogenesis. Our in-depth dynamic proteomic analysis indicated the comprehensive and dynamic regulation mechanism during hPDLSC osteogenesis.
HostingRepository	PRIDE
AnnounceDate	2021-02-11
AnnouncementXML	Submission_2021-02-11_01:51:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianjia Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-14 02:45:29	ID requested
⏵ 1	2021-02-11 01:51:01	announced

Li J, Wang Z, Huang X, Wang Z, Chen Z, Wang R, Chen Z, Liu W, Wu B, Fang F, Qiu W, Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation. Stem Cell Res Ther, 12(1):98(2021) [pubmed]

submitter keyword: Human, Periodontal, Dynamic expression, TMT

Wei Qiu
contact affiliation	Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, China, 510515
contact email	qiuweiandmj@163.com
lab head
Jianjia Li
contact affiliation	Department of Stomatology, Nanfang Hospital, Southern Medical University
contact email	kathyli199304@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD020908

PRIDE project URI

• PRIDE
  1. PXD020908
     1. Label: PRIDE project
     2. Name: Dynamic proteomic profiling of human periodontal ligament stem cells during osteogenic differentiation